A new anticancer compound, Oblongifolin C, inhibits tumor growth and promotes apoptosis in HeLa cells through bax activation

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	A new anticancer compound, Oblongifolin C, inhibits tumor growth and promotes apoptosis in HeLa cells through bax activation
	Feng, Chao 2; Zhou, Li-Ying 2; Yu, Ting 1; Xu, Gang3 ; Tian, Hong-Lei 4; Xu, Jin-Jie 2; Xu, Hong-Xi 5; Luo, Kathy Qian 1
刊名	INTERNATIONAL JOURNAL OF CANCER
	2012-09-15
卷号	131 期号:6 页码:1445-1454
关键词	anticancer drug discovery herbal medicine oblongifolin C apoptosis Bax
ISSN号	0020-7136
通讯作者	Luo, KQ (reprint author), Nanyang Technol Univ, Div Bioengn, Sch Chem & Biomed Engn, 70 Nanyang Dr, Singapore 637457, Singapore.,xuhongxi88@gmail.com ; kluo@ntu.edu.sg
英文摘要	Oblongifolin C (OC) was identified as a potent apoptosis inducer from an herbal plant, Garcinia yunnanensis, during our previous bioassay-guided drug screening. In this study, we investigated the signaling pathways through which OC activated apoptosis in HeLa cells. We also compared the IC50 values of OC with that of etoposide, paclitaxel and vinblastine in multiple cancer cell lines including HER2 and P-glycoprotein overexpressing cells. In addition, the in vivo antitumor effect of OC was studied in nude mice model. Our results showed that OC induced a caspase-dependent apoptosis by triggering a series of events in HeLa cells including Bax translocation, cytochrome c release, caspase-3 activation, chromosome fragmentation followed by caspase-8 activation, Bid cleavage and eventually cell death. Addition of a pan-caspase inhibitor or overexpression of an anti-apoptotic protein, Bcl-xL, prevented OC-induced cell death. Moreover, OC exhibited a wide anticancer spectrum in multiple cancer cell lines with comparable IC50 values, regardless of the expression levels of HER2 and P-glycoprotein. In contrast, the IC50 values of three clinical anticancer drugs, etoposide, paclitaxel and vinblastine were significantly elevated in HER2 and/or P-glycoprotein overexpressing cells. Furthermore, OC showed a similar antitumor effect but lower general toxicity than etoposide against xenografted human tumors in nude mice model. All these data suggested that OC is a promising apoptosis inducer with the potential to be developed into a clinical anticancer drug.
类目[WOS]	Oncology
研究领域[WOS]	Oncology
关键词[WOS]	CYTOCHROME-C ; DRUG SCREEN ; GARCINIA-SMEATHMANNII ; CANCER ; RESISTANCE ; CASPASES ; PROTEIN ; DEATH ; BENZOPHENONES ; DERIVATIVES
收录类别	SCI
资助信息	Academic Research Fund (AcRF) Tier 1 grant of the Minister of Education of Singapore [M52120104]; National Natural Science Foundation of China [81173485]
语种	英语
WOS记录号	WOS:000306735700045
公开日期	2012-11-30
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn:8080/handle/151853/15692]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.Nanyang Technol Univ, Div Bioengn, Sch Chem & Biomed Engn, Singapore 637457, Singapore 2.Hong Kong Univ Sci & Technol, Dept Chem & Biomol Engn, Hong Kong, Hong Kong, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Kunming, Yunnan, Peoples R China 4.Hong Kong Univ Sci & Technol, Dept Biochem, Hong Kong, Hong Kong, Peoples R China 5.Shanghai Univ Tradit Chinese Med, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Feng, Chao,Zhou, Li-Ying,Yu, Ting,et al. A new anticancer compound, Oblongifolin C, inhibits tumor growth and promotes apoptosis in HeLa cells through bax activation[J]. INTERNATIONAL JOURNAL OF CANCER,2012,131(6):1445-1454.
APA	Feng, Chao.,Zhou, Li-Ying.,Yu, Ting.,Xu, Gang.,Tian, Hong-Lei.,...&Luo, Kathy Qian.(2012).A new anticancer compound, Oblongifolin C, inhibits tumor growth and promotes apoptosis in HeLa cells through bax activation.INTERNATIONAL JOURNAL OF CANCER,131(6),1445-1454.
MLA	Feng, Chao,et al."A new anticancer compound, Oblongifolin C, inhibits tumor growth and promotes apoptosis in HeLa cells through bax activation".INTERNATIONAL JOURNAL OF CANCER 131.6(2012):1445-1454.