Chemometric analysis of metabolism disorders in blood plasma of S180 and H22 tumor-bearing mice by high performance liquid chromatography- diode array detection.

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Chemometric analysis of metabolism disorders in blood plasma of S180 and H22 tumor-bearing mice by high performance liquid chromatography- diode array detection.
	Liu Y(刘芸); Sun XM(孙小明); Di DL(邸多隆); Di DL(邸多隆)
刊名	Journal of Chemometrics
	2011
关键词	metabonomics metabolic fingerprints multivariate statistical analysis biomarkers high performance liquid chromatography-diode array detector
ISSN号	0886-9383
通讯作者	邸多隆
中文摘要	The aim of this paper is to characterize metabolism disorders in Kunming mice induced by S180 and H22 tumor cells.Metabolic fingerprint based on high performance liquid chromatography-diode array detector (HPLC-DAD) was developed to map the disturbed metabolic responses. In vivo testing of the antitumor activity of paclitaxel (Taxol) was carried out by inhibiting the growth of S180 and H22 tumor cells. Based on 27 common peaks, principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used to distinguish the abnormal from control and to find significant endogenous compounds (SECs) which have significant contributions to classification.The tumor growth inhibition ratios (TIRs) of Taxol groups were used to validate the predictive accuracies of the PLS-DA models. The predictive accuracies of PLS-DA models for S180 and H22 tumor model groups were 97.6 and 100%,respectively. Nine (S180) and seven (H22) SECs were discovered, including uric acid and cytidine. In addition, the correlations between relative tumor weights (RTWs) and chromatographic data for the SECs were significant (p<0.05). Investigations on the stability and precision of the established metabolic fingerprints demonstrate that the experiment is well controlled and reliable. This work shows that the platform of HPLC-DAD coupled with chemometric methods provides a promising method for the study of metabolism disorders induced by tumor cells.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the “Hundred Talents Program” of Chinese Academy of Sciences (CAS) in 2007;the National Natural Sciences Foundation of China (NSFC no. 20775083)
语种	英语
公开日期	2012-11-26
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/2228]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
通讯作者	Di DL(邸多隆); Di DL(邸多隆)
推荐引用方式 GB/T 7714	Liu Y,Sun XM,Di DL,et al. Chemometric analysis of metabolism disorders in blood plasma of S180 and H22 tumor-bearing mice by high performance liquid chromatography- diode array detection.[J]. Journal of Chemometrics,2011.
APA	刘芸,孙小明,邸多隆,&邸多隆.(2011).Chemometric analysis of metabolism disorders in blood plasma of S180 and H22 tumor-bearing mice by high performance liquid chromatography- diode array detection..Journal of Chemometrics.
MLA	刘芸,et al."Chemometric analysis of metabolism disorders in blood plasma of S180 and H22 tumor-bearing mice by high performance liquid chromatography- diode array detection.".Journal of Chemometrics (2011).