Biocompatible magnetic cellulose-chitosan hybrid gel microspheres reconstituted from ionic liquids for enzyme immobilization | |
Liu, Zhen; Wang, Haisong; Li, Bin; Liu, Chao; Jiang, Yijun; Yu, Guang; Mu, Xindong | |
刊名 | JOURNAL OF MATERIALS CHEMISTRY |
2012 | |
卷号 | 22期号:30页码:15085-15091 |
中文摘要 | Magnetic Fe3O4–cellulose–chitosan hybrid gel microspheres were prepared by sol–gel transition technology using ionic liquids as solvent for cellulose and chitosan dissolution and regeneration. The synthesized microspheres were characterized by XRD, FTIR, SEM, and vibrating sample magnetometer (VSM). The XRD and FTIR results showed that chitosan and cellulose had been successfully coated onto the surface of Fe3O4 after the preparation. SEM presented that the synthesized microspheres were regular sphere with a mean diameter of about 10 μm. Furthermore, it was found that glucose oxidase (GOx) could be successfully immobilized on the hybrid gel microspheres via glutaraldehyde technique. The immobilized enzyme presented higher thermostability, wider range of pH optima and improved storage stability in comparison with free GOx. The glucose conversion could reach 91.5% within 4 h and retained 84.2% of the initial activity after 15 cycles of use. These results demonstrate their potential applications in the field of biocatalysis. |
英文摘要 | Magnetic Fe3O4-cellulose-chitosan hybrid gel microspheres were prepared by sol-gel transition technology using ionic liquids as solvent for cellulose and chitosan dissolution and regeneration. The synthesized microspheres were characterized by XRD, FTIR, SEM, and vibrating sample magnetometer (VSM). The XRD and FTIR results showed that chitosan and cellulose had been successfully coated onto the surface of Fe3O4 after the preparation. SEM presented that the synthesized microspheres were regular sphere with a mean diameter of about 10 mm. Furthermore, it was found that glucose oxidase (GOx) could be successfully immobilized on the hybrid gel microspheres via glutaraldehyde technique. The immobilized enzyme presented higher thermostability, wider range of pH optima and improved storage stability in comparison with free GOx. The glucose conversion could reach 91.5% within 4 h and retained 84.2% of the initial activity after 15 cycles of use. These results demonstrate their potential applications in the field of biocatalysis. |
学科主题 | 绿色化学催化 |
WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
类目[WOS] | Chemistry, Physical ; Materials Science, Multidisciplinary |
研究领域[WOS] | Chemistry ; Materials Science |
关键词[WOS] | X-RAY-DIFFRACTION ; AQUEOUS-SOLUTION ; CRYSTALLINE-STRUCTURE ; GLUCOSE-OXIDASE ; DISSOLUTION ; NANOPARTICLES ; COMPOSITES ; STABILITY ; FIBERS ; FILMS |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000306215900041 |
公开日期 | 2012-11-16 |
内容类型 | 期刊论文 |
源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1399] |
专题 | 青岛生物能源与过程研究所_绿色化学催化团队 |
作者单位 | Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Zhen,Wang, Haisong,Li, Bin,et al. Biocompatible magnetic cellulose-chitosan hybrid gel microspheres reconstituted from ionic liquids for enzyme immobilization[J]. JOURNAL OF MATERIALS CHEMISTRY,2012,22(30):15085-15091. |
APA | Liu, Zhen.,Wang, Haisong.,Li, Bin.,Liu, Chao.,Jiang, Yijun.,...&Mu, Xindong.(2012).Biocompatible magnetic cellulose-chitosan hybrid gel microspheres reconstituted from ionic liquids for enzyme immobilization.JOURNAL OF MATERIALS CHEMISTRY,22(30),15085-15091. |
MLA | Liu, Zhen,et al."Biocompatible magnetic cellulose-chitosan hybrid gel microspheres reconstituted from ionic liquids for enzyme immobilization".JOURNAL OF MATERIALS CHEMISTRY 22.30(2012):15085-15091. |
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