Quantitative analysis of fatty-acid-based biofuels produced by wild-type and genetically engineered cyanobacteria by gas chromatography-mass spectrometry | |
Guan, Wenna1; Zhao, Hui2; Lu, Xuefeng2; Wang, Cong1; Yang, Menglong1; Bai, Fali1 | |
刊名 | JOURNAL OF CHROMATOGRAPHY A |
2011-11-11 | |
卷号 | 1218期号:45页码:8289-8293 |
关键词 | Fatty-acid-based biofuels Gas chromatography-mass spectrometry (GC-MS) Microalgae Genetically engineered cyanobacteria |
中文摘要 | Simple and rapid quantitative determination of fatty-acid-based biofuels is greatly important for the study of genetic engineering progress for biofuels production by microalgae. Ideal biofuels produced from biological systems should be chemically similar to petroleum, like fatty-acid-based molecules including free fatty acids, fatty acid methyl esters, fatty acid ethyl esters, fatty alcohols and fatty alkanes. This study founded a gas chromatography-mass spectrometry (GC-MS) method for simultaneous quantification of seven free fatty acids, nine fatty acid methyl esters, five fatty acid ethyl esters, five fatty alcohols and three fatty alkanes produced by wild-type Synechocystis PCC 6803 and its genetically engineered strain. Data obtained from GC-MS analyses were quantified using internal standard peak area comparisons. The linearity, limit of detection (LOD) and precision (RSD) of the method were evaluated. The results demonstrated that fatty-acid-based biofuels can be directly determined by GC-MS without derivation. Therefore, rapid and reliable quantitative analysis of fatty-acid-based biofuels produced by wild-type and genetically engineered cyanobacteria can be achieved using the GC-MS method founded in this work. |
英文摘要 | Simple and rapid quantitative determination of fatty-acid-based biofuels is greatly important for the study of genetic engineering progress for biofuels production by microalgae. Ideal biofuels produced from biological systems should be chemically similar to petroleum, like fatty-acid-based molecules including free fatty acids, fatty acid methyl esters, fatty acid ethyl esters, fatty alcohols and fatty alkanes. This study founded a gas chromatography-mass spectrometry (GC-MS) method for simultaneous quantification of seven free fatty acids, nine fatty acid methyl esters, five fatty acid ethyl esters, five fatty alcohols and three fatty alkanes produced by wild-type Synechocystis PCC 6803 and its genetically engineered strain. Data obtained from GC-MS analyses were quantified using internal standard peak area comparisons. The linearity, limit of detection (LOD) and precision (RSD) of the method were evaluated. The results demonstrated that fatty-acid-based biofuels can be directly determined by GC-MS without derivation. Therefore, rapid and reliable quantitative analysis of fatty-acid-based biofuels produced by wild-type and genetically engineered cyanobacteria can be achieved using the GC-MS method founded in this work. (C) 2011 Elsevier B.V. All rights reserved. |
学科主题 | 生物代谢工程 |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
关键词[WOS] | BOTRYOCOCCUS-BRAUNII ; BIODIESEL PRODUCTION ; LIGHT-INTENSITY ; MICROALGAE ; GROWTH ; PHASE ; CULTIVATION ; PROFILES ; GALBANA ; BIOMASS |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000296937400018 |
公开日期 | 2012-11-11 |
内容类型 | 期刊论文 |
源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/1299] |
专题 | 青岛生物能源与过程研究所_微生物代谢工程团队 |
作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Publ Lab Bioenergy & Biofuels, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China |
推荐引用方式 GB/T 7714 | Guan, Wenna,Zhao, Hui,Lu, Xuefeng,et al. Quantitative analysis of fatty-acid-based biofuels produced by wild-type and genetically engineered cyanobacteria by gas chromatography-mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2011,1218(45):8289-8293. |
APA | Guan, Wenna,Zhao, Hui,Lu, Xuefeng,Wang, Cong,Yang, Menglong,&Bai, Fali.(2011).Quantitative analysis of fatty-acid-based biofuels produced by wild-type and genetically engineered cyanobacteria by gas chromatography-mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1218(45),8289-8293. |
MLA | Guan, Wenna,et al."Quantitative analysis of fatty-acid-based biofuels produced by wild-type and genetically engineered cyanobacteria by gas chromatography-mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1218.45(2011):8289-8293. |
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