Ligand fishing with functionalized magnetic nanoparticles coupled with mass spectrometry for herbal medicine analysis

	Ligand fishing with functionalized magnetic nanoparticles coupled with mass spectrometry for herbal medicine analysis
	Qing, Lin-Sen ; Xue, Ying ; Deng, Wen-Long ; Liao, Xun ; Xu, Xue-Min ; Li, Bo-Gang ; Liu, Yi-Ming
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2011
卷号	399 期号:3 页码:1223-1231
关键词	Magnetic nanoparticles Ligand fishing ESI-MS/MS Quality assessment of herbal medicines Dioscorea panthaica
ISSN号	1618-2642
通讯作者	Liao, Xun ; Liu, Yi-Ming
产权排序	1
英文摘要	The chemical composition of herbal medicines is very complex, and their therapeutic effects are determined by multi-components with sophisticated synergistic and/or suppressive actions. Therefore, quality control of herbal medicines has been a formidable challenge. In this work, we describe a fast analytical method that can be used for quality assessment of herbal medicines. The method is based on ligand fishing using human-serum-albumin-functionalized magnetic nanoparticles (HSA-MNPs) and mass spectrometry. To demonstrate the applicability of the proposed method, eight samples of Dioscorea panthaica were analyzed. The sampled plants were of both wild and cultivated origins. They grew at different geographical locations and were harvested at different times. The ligands bound to HSA-MNPs were isolated from the plant extracts and detected by using direct infusion electrospray ionization mass spectrometry (DI-ESI-MS). Chemical identity has been confirmed for five of the ligands isolated. From more than 15 peaks in the ESI-MS spectrum, 11 common peaks were selected for calculating the correlation coefficient and cosine ratio. The values of correlation coefficient and cosine ratio were >0.9824 and >0.9988, respectively, for all the samples tested. The results indicated a high level of similarity among the eight D. panthaica samples. Compared with chromatographic fingerprint analysis, the proposed HSA-MNP-based DI-ESI-MS/MS approach was not only fast and easy to carry out but also biologicalactivity-oriented, promising a more effective data interpretation and thus reliable assessment conclusions.
学科主题	Biochemistry & Molecular Biology; Chemistry
收录类别	SCI
资助信息	National Natural Science Foundation of China[20872137/B020402]; US National Institutes of Health[SC1 GM089557]
语种	英语
WOS记录号	WOS:000286599100022
公开日期	2012-11-09
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/23567]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Qing, Lin-Sen,Xue, Ying,Deng, Wen-Long,et al. Ligand fishing with functionalized magnetic nanoparticles coupled with mass spectrometry for herbal medicine analysis[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2011,399(3):1223-1231.
APA	Qing, Lin-Sen.,Xue, Ying.,Deng, Wen-Long.,Liao, Xun.,Xu, Xue-Min.,...&Liu, Yi-Ming.(2011).Ligand fishing with functionalized magnetic nanoparticles coupled with mass spectrometry for herbal medicine analysis.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,399(3),1223-1231.
MLA	Qing, Lin-Sen,et al."Ligand fishing with functionalized magnetic nanoparticles coupled with mass spectrometry for herbal medicine analysis".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 399.3(2011):1223-1231.