Colorimetric detection of mercury, lead and copper ions simultaneously using protein-functionalized gold nanoparticles

	Colorimetric detection of mercury, lead and copper ions simultaneously using protein-functionalized gold nanoparticles
	Guo, Yongming ; Wang, Zhuo ; Qu, Weisi ; Shao, Huawu ; Jiang, Xingyu
刊名	BIOSENSORS & BIOELECTRONICS
	2011
卷号	26 期号:10 页码:4064-4069
关键词	Gold nanoparticles Mercury ions Lead ions Copper ions Protein Colorimetric detection
ISSN号	0956-5663
通讯作者	Shao, Huawu ; Jiang, Xingyu
产权排序	1
英文摘要	A simple, cost-effective and rapid colorimetric method for any or all of Hg2+ Pb2+ and Cu2+ detection using papain-functionalized gold nanoparticles (P-AuNPs) has been developed. Papain is a protein with seven cystein residues, which can selectively bind with Hg2+, Pb2+ and Cu2+. We functionalized gold nanoparticles with papain. The P-AuNPs could be used to simultaneously detect Hg2+, Pb2+ and Cu2+, and showed different responses to the three ions in an aqueous solution based on the aggregation-induced color change of gold nanoparticles. The P-AuNPs displayed the most obvious response to mercury ions in water in contrast to lead and copper ions, and the real water sample analysis verified the conclusion. The sensitivity of the detection system was influenced by the pH of the P-AuNPs solution, the concentration of P-AuNPs and the size of gold nanoparticles, and we found that larger gold nanoparticles contributed to more sensitive results. The detection system can detect as low as 200 nM Hg2+, Pb2+ or Cu2+ using 42 nm gold nanoparticles. We expect our approach to have wide-ranging applications in the developing region for monitoring water quality in some areas. (C) 2011 Elsevier B.V. All rights reserved.
学科主题	Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics
收录类别	SCI
资助信息	National Natural Science Foundation of China[90813032, 20890020, 21025520]; Chinese Academy of Sciences[KJCX2-YW-M15]; Scientific Research Foundation for the Returned Overseas Chinese Scholars, the Ministry of Science and Technology[2009CB30001, 2011CB933201]; Ministry of Human Resources and Social Security of the People's Republic of China
语种	英语
WOS记录号	WOS:000291904000014
公开日期	2012-11-09
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/23542]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Guo, Yongming,Wang, Zhuo,Qu, Weisi,et al. Colorimetric detection of mercury, lead and copper ions simultaneously using protein-functionalized gold nanoparticles[J]. BIOSENSORS & BIOELECTRONICS,2011,26(10):4064-4069.
APA	Guo, Yongming,Wang, Zhuo,Qu, Weisi,Shao, Huawu,&Jiang, Xingyu.(2011).Colorimetric detection of mercury, lead and copper ions simultaneously using protein-functionalized gold nanoparticles.BIOSENSORS & BIOELECTRONICS,26(10),4064-4069.
MLA	Guo, Yongming,et al."Colorimetric detection of mercury, lead and copper ions simultaneously using protein-functionalized gold nanoparticles".BIOSENSORS & BIOELECTRONICS 26.10(2011):4064-4069.