Flavokawain B induces apoptosis of human oral adenoid cystic cancer ACC-2 cells via upregulation of Bim and down-regulation of Bcl-2 expression

	Flavokawain B induces apoptosis of human oral adenoid cystic cancer ACC-2 cells via upregulation of Bim and down-regulation of Bcl-2 expression
	Zhao, Xi ; Chao, Yong-Lie ; Wan, Qian-Bing ; Chen, Xin-Min ; Su, Peng ; Sun, Jian ; Tang, Yaxiong
刊名	CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
	2011
卷号	89 期号:12 页码:875-882
关键词	flavokawain B (FKB) apoptosis Bcl-2 Bim ACC-2
ISSN号	0008-4212
通讯作者	Tang, YX ; Sun, Jian
产权排序	2
英文摘要	Novel effective drugs are still urgently needed in the prevention and treatment of oral adenoid cystic carcinoma (ACC). In this study, we have assessed the antitumor potential and molecular mechanisms of flavokawain B (FKB) as a kava chalcone on the ACC-2 cell line in vitro. The results demonstrated that FKB could significantly inhibit the cell proliferation of ACC-2 in a dose-dependent manner that was associated with induced apoptosis and cell cycle G2-M arrest, and the half maximal inhibitory concentration (IC(50)) of flavokawain-B treatment for 48 h was estimated to be 4.69 +/- 0.43 mu mol/L. Mechanistically, FKB could induce the release of cytochrome c from mitochondria into the cytosol, and activate the cleavage of caspase-3 and, eventually, the poly(ADP-ribose) polymerase (PARP), in a dose-dependent manner, leading to marked apoptotic effect of ACC-2 cells. The apoptotic action of FKB was associated with the increased expression of proapoptotic proteins: Bim, Bax, Bak and a decreased expression of antiapoptotic Bcl-2. Among them, Bim expression was significantly induced by FKB, and knockdown of Bim expression by short-hairpin RNAs attenuated the inhibitory effect induced by FKB on ACC-2 cells. These results suggest Bim may be one of the potential transcriptional targets, and suggests the potential usefulness of FKB for the prevention and treatment of ACC.
学科主题	Pharmacology & Pharmacy; Physiology
收录类别	SCI
资助信息	National Natural Science Foundation of China[81072662, 91013006]
语种	英语
WOS记录号	WOS:000298441800002
公开日期	2012-11-09
内容类型	期刊论文
源URL	[http://210.75.237.14/handle/351003/23518]
专题	成都生物研究所_天然产物研究
推荐引用方式 GB/T 7714	Zhao, Xi,Chao, Yong-Lie,Wan, Qian-Bing,et al. Flavokawain B induces apoptosis of human oral adenoid cystic cancer ACC-2 cells via upregulation of Bim and down-regulation of Bcl-2 expression[J]. CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY,2011,89(12):875-882.
APA	Zhao, Xi.,Chao, Yong-Lie.,Wan, Qian-Bing.,Chen, Xin-Min.,Su, Peng.,...&Tang, Yaxiong.(2011).Flavokawain B induces apoptosis of human oral adenoid cystic cancer ACC-2 cells via upregulation of Bim and down-regulation of Bcl-2 expression.CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY,89(12),875-882.
MLA	Zhao, Xi,et al."Flavokawain B induces apoptosis of human oral adenoid cystic cancer ACC-2 cells via upregulation of Bim and down-regulation of Bcl-2 expression".CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY 89.12(2011):875-882.