Evaluation of the biocompatibile ionic liquid 1-methyl-3-methylimidazolium dimethylphosphite pretreatment of corncob for improved saccharification

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 材料生物技术研究中心

	Evaluation of the biocompatibile ionic liquid 1-methyl-3-methylimidazolium dimethylphosphite pretreatment of corncob for improved saccharification
	Mo Xian
刊名	Applied Microbiology and Biotechnology
	2010
卷号	87 期号:1 页码:117-26
关键词	Corn cob - Biocompatibility - Ionic liquids pretreatment - 1-Methyl-3-methylimidazolium dimethylphosphite - Enzymatic hydrolysis - Microbial lipid
中文摘要	Ionic liquid (IL) pretreatment of lignocellulose materials is a promising process in biomass conversion to renewable biofuel. More in-depth research involving environment-friendly IL is much needed to explore pretreatment green route. In our case, IL 1-methyl-3-methylimidazolium dimethylphosphite ([Mmim]DMP) was chosen as an environment-friendly solvent to pretreat corn cob in view of its biocompatibility with both lignocellulose solubility and cellulase activity. The pretreatment/saccharification process and in situ saccharification process involving [Mmim]DMP were efficiently performed in bioconversion of corn cob to sugars, and more than 70% saccharification rates were obtained. Furthermore, the fermentability of reducing sugars obtained from the hydrolyzates was evaluated using Rhodococcus opacus strain ACCC41043 (R. opacus). High lipid production 41–43% of cell dry matter was obtained after 30 h of cultivation. GC/MS analysis indicated that lipids from R. opacus contained mainly long-chain fatty acids with four major constituent/oleic acid, stearic acid, palmitic acid, palmitoleic acid which are good candidates for biodiesel. These elucidated that corn cob pretreated by IL [Mmim]DMP did not bring negative effects on saccharification, cell growth, and accumulation of lipid of R. opacus. In conclusion, the IL [Mmim]DMP shows promise as green pretreatment solvent for cellulosic materials.
学科主题	生物基化学品
收录类别	SCI
语种	英语
公开日期	2012-11-07
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1106]
专题	青岛生物能源与过程研究所_材料生物技术研究中心
推荐引用方式 GB/T 7714	Mo Xian. Evaluation of the biocompatibile ionic liquid 1-methyl-3-methylimidazolium dimethylphosphite pretreatment of corncob for improved saccharification[J]. Applied Microbiology and Biotechnology,2010,87(1):117-26.
APA	Mo Xian.(2010).Evaluation of the biocompatibile ionic liquid 1-methyl-3-methylimidazolium dimethylphosphite pretreatment of corncob for improved saccharification.Applied Microbiology and Biotechnology,87(1),117-26.
MLA	Mo Xian."Evaluation of the biocompatibile ionic liquid 1-methyl-3-methylimidazolium dimethylphosphite pretreatment of corncob for improved saccharification".Applied Microbiology and Biotechnology 87.1(2010):117-26.