Ca2+-independent but voltage-dependent secretion in mammalian dorsal root ganglion neurons

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	Ca2+-independent but voltage-dependent secretion in mammalian dorsal root ganglion neurons
	Zhang, C ; Zhou, Z
刊名	NATURE NEUROSCIENCE
	2002
卷号	5 期号:5 页码:425-430
关键词	ADRENAL CHROMAFFIN CELLS TRANSMITTER RELEASE AMPEROMETRIC DETECTION QUANTAL SECRETION EXOCYTOSIS CATECHOLAMINES COMPETENT KINETICS PATHWAYS CALCIUM
ISSN号	1097-6256
通讯作者	Zhou, Z (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,zzhou@ion.ac.cn
英文摘要	We have investigated the Ca2+ dependence of vesicular secretion from the soma of dorsal root ganglion (DRG) neurons, which secrete neuropeptides by exocytosis of dense-core vesicles. In patch-clamped somata of rat DRG neurons, we found a depolarization-induced membrane capacitance increase (DeltaC(m)) in the absence of extracellular Ca2+ and in the presence of a Ca2+ chelator (BAPTA) in the intracellular solution. Depletion of internal Ca2+ stores by thapsigargin in the Ca2+-free bath also did not block the DeltaC(m), indicating that Ca2+ release from internal Ca2+ stores may not have been involved. Furthermore, the Ca2+-independent DeltaC(m) was blocked by whole-cell dialysis with tetanus toxin and was accompanied by pulsatile secretion of false transmitters, as detected by amperometric measurements. These results indicate the existence of Ca2+-independent but voltage-dependent vesicular secretion (CIVDS) in a mammalian sensory neuron.
学科主题	Neurosciences & Neurology
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/2061]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Zhang, C,Zhou, Z. Ca2+-independent but voltage-dependent secretion in mammalian dorsal root ganglion neurons[J]. NATURE NEUROSCIENCE,2002,5(5):425-430.
APA	Zhang, C,&Zhou, Z.(2002).Ca2+-independent but voltage-dependent secretion in mammalian dorsal root ganglion neurons.NATURE NEUROSCIENCE,5(5),425-430.
MLA	Zhang, C,et al."Ca2+-independent but voltage-dependent secretion in mammalian dorsal root ganglion neurons".NATURE NEUROSCIENCE 5.5(2002):425-430.