A novel scorpion toxin blocking small conductance Ca2+ activated K+ channel

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	A novel scorpion toxin blocking small conductance Ca2+ activated K+ channel
	Xu, CQ ; He, LL ; Brone, B ; Martin-Eauclaire, MF ; Van Kerkhove, E ; Zhou, Z ; Chi, CW
刊名	TOXICON
	2004
卷号	43 期号:8 页码:961-971
关键词	SK channel amino acid sequence gene cloning phylogenetic tree ADRENAL CHROMAFFIN CELLS BUTHUS-MARTENSI KARSCH UNPAIRED MEDIAN NEURONS POTASSIUM CHANNELS GATING MODIFIER LEIUROTOXIN-I ION CHANNELS CALCIUM VENOM APAMIN
ISSN号	0041-0101
通讯作者	Chi, CW (reprint author), Chinese Acad Sci, Key Lab Proteom, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,chi@sunm.shcnc.ac.cn
英文摘要	Small conductance calcium activated potassium channels (SK) are crucial in the regulation of cell firing frequency in the nervous system and other tissues. In the present work, a novel SK channel blocker, designated BmSKTx1, was purified from the scorpion Buthus martensi Karsh venom. The sequence of the N-terminal 22 amino acid residues was determined by Edman degradation. Using this sequence information, the full-length cDNA and genomic gene of BmSKTx1 were cloned and sequenced. By these analyses, BmSKTx1 was found to be a peptide composed of 31 amino acid residues with three disulfide bonds. It shared little sequence homology with other known scorpion alpha-KTxs but showed close relationship with SK channel blockers in the phylogenetic tree. According to the previous nomenclature, BmSKTx1 was classified as alpha-KTx14.1. We examined the effects of BmSKTx1 on different ion channels of rat adrenal chromaffin cells (RACC) and locust dorsal unpaired median (DUM) neurons. BmSKTx1 selectively inhibited apamin-sensitive SK currents in RACC with K-d of 0.72 p,M and Hill coefficient of 2.2. And it had no effect on Na+, Ca2+, Kv, and BK currents in DUM neuron, indicating that BmSKTx1 was a selective SK toxin. (C) 2004 Elsevier Ltd. All rights reserved.
学科主题	Pharmacology & Pharmacy ; Toxicology
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1957]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Xu, CQ,He, LL,Brone, B,et al. A novel scorpion toxin blocking small conductance Ca2+ activated K+ channel[J]. TOXICON,2004,43(8):961-971.
APA	Xu, CQ.,He, LL.,Brone, B.,Martin-Eauclaire, MF.,Van Kerkhove, E.,...&Chi, CW.(2004).A novel scorpion toxin blocking small conductance Ca2+ activated K+ channel.TOXICON,43(8),961-971.
MLA	Xu, CQ,et al."A novel scorpion toxin blocking small conductance Ca2+ activated K+ channel".TOXICON 43.8(2004):961-971.