Downregulation of KCC2 following LTP-contributes to EPSP-spike potentiation in rat hippocampus

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	Downregulation of KCC2 following LTP-contributes to EPSP-spike potentiation in rat hippocampus
	Wang, W ; Gong, N ; Xu, TL(徐天乐)
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2006
卷号	343 期号:4 页码:1209-1215
关键词	KCC2 LTP GABAergic inhibition NMDA receptor E-S potentiation LONG-TERM POTENTIATION GAMMA-AMINOBUTYRIC-ACID COTRANSPORTER KCC2 GABAERGIC SYNAPSES GABA(A) RECEPTORS PYRAMIDAL NEURONS CA1 NEURONS INDUCTION STIMULATION INHIBITION
ISSN号	0006-291X
通讯作者	Xu, TL (reprint author), Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China,tlxu@ion.ac.cn
英文摘要	GABAergic synaptic inhibition plays a critical role in regulating long-term potentiation (LTP) of glutamatergic synaptic transmission and circuit output. The K+-Cl- cotransporter 2 (KCC2) is an important factor in determining inhibitory GABAergic synaptic strength besides the contribution of GABA(A) receptor. Although much knowledge has been gained regarding activity-dependent downregulation of KCC2 in many pathological conditions, the potential change and contribution of KCC2 in UP expression is still unknown. In this study, we found that down regulation of KCC2 was accompanied with the occurrence of LTP but not that of long-term depression in hippocampal CA1 region. Meanwhile, KCC2 level in CA3/DG and adjacent cortex was stable in the process of LTP expression in Schaffer collateral synapses. Blockade of NMDA receptor with APV not only prevented LTP induction also abolished the reduction of KCC2. Furthermore, the inhibition of KCC2 function with furosemide directly induced EPSP-spike (E-S) potentiation, an important component of UP in hippocampus. The present data suggest a novel mechanism that LTP formation is accompanied by the downregulation of KCC2, which is underlying GABAergic strength and most likely contributes to the E-S potentiation following LTP. (c) 2006 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Biophysics
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1823]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Wang, W,Gong, N,Xu, TL. Downregulation of KCC2 following LTP-contributes to EPSP-spike potentiation in rat hippocampus[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2006,343(4):1209-1215.
APA	Wang, W,Gong, N,&Xu, TL.(2006).Downregulation of KCC2 following LTP-contributes to EPSP-spike potentiation in rat hippocampus.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,343(4),1209-1215.
MLA	Wang, W,et al."Downregulation of KCC2 following LTP-contributes to EPSP-spike potentiation in rat hippocampus".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 343.4(2006):1209-1215.