Identification of acid-sensing ion channels in adenoid cystic carcinomas

CORC > 上海神经科学研究所 > 中国科学院上海生命科学研究院神经科学研究所 > 神经所(总)

	Identification of acid-sensing ion channels in adenoid cystic carcinomas
	Ye, Jin-Hai ; Gao, Jun ; Wu, Yu-Nong ; Hu, Yong-Jie ; Zhang, Chen-Ping ; Xu, Tian-Le(徐天乐)
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2007
卷号	355 期号:4 页码:986-992
关键词	acid-sensing ion channels adenoid cystic carcinomas patch clamp RT-PCR western blotting MUTATIONS CAUSING NEURODEGENERATION MAMMALIAN DEGENERIN MDEG GATED NA+ CHANNEL CELL-LINE CAENORHABDITIS-ELEGANS POTASSIUM CHANNEL H+ CALCIUM PH LYMPHOCYTES
ISSN号	0006-291X
通讯作者	Zhang, CP (reprint author), Shanghai Jiao Tong Univ, Dept Oral & Maxillofacial Surg, Sch Stomatol,Shanghai Res Inst Stomatol, Affiliated Peoples Hosp 9, Shanghai 200011, Peoples R China,yjh98001@sjtu.edu.cn
英文摘要	Tissue acidosis is an important feature of tumor. The response of adenoid cystic carcinoma (ACC) cells to acidic solution was studied using whole-cell patch-clamp recording in the current study. An inward, amiloride-sensitive Na+ current was identified in cultured ACC2 cells while not in normal human salivary gland epithelial cells. Electrophysiological and pharmacological properties of the currents suggest that heteromeric acid-sensing ion channels (ASICs) containing 2a and 3 may be responsible for the proton-induced currents in the majority of ACC-2 cells. Consistent with it, analyses of RT-PCR and Western blotting demonstrated the presences of ASIC2a and 3 in ACC-2 cells. Furthermore, we observed the enhanced expression of ASIC2a and 3 in the sample of ACC tissues. These results indicate that the functional expression of ASICs is characteristic feature of ACC cells. (c) 2007 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Biophysics
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1776]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Ye, Jin-Hai,Gao, Jun,Wu, Yu-Nong,et al. Identification of acid-sensing ion channels in adenoid cystic carcinomas[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2007,355(4):986-992.
APA	Ye, Jin-Hai,Gao, Jun,Wu, Yu-Nong,Hu, Yong-Jie,Zhang, Chen-Ping,&Xu, Tian-Le.(2007).Identification of acid-sensing ion channels in adenoid cystic carcinomas.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,355(4),986-992.
MLA	Ye, Jin-Hai,et al."Identification of acid-sensing ion channels in adenoid cystic carcinomas".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 355.4(2007):986-992.