Rapsyn interaction with calpain stabilizes AChR clusters at the neuromuscular junction

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	Rapsyn interaction with calpain stabilizes AChR clusters at the neuromuscular junction
	Luo, Zhen-Ge
刊名	NEURON
	2007
卷号	55 期号:2 页码:247-260
关键词	CYCLIN-DEPENDENT KINASE-5 ACETYLCHOLINE-RECEPTOR CLUSTERS ALTERNATIVELY SPLICED ISOFORMS SYNAPSE FORMATION CDK5 ACTIVITY RAT MYOTUBES NULL MICE AGRIN P25 PROTEIN
ISSN号	0896-6273
通讯作者	Luo, ZG (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,zgluo@ion.ac.cn
英文摘要	Agrin induces, whereas acetylcholine (ACh) disperses, ACh receptor (AChR) clusters during neuromuscular synaptogenesis. Such counteractive interaction leads to eventual dispersal of nonsynaptic AChR-rich sites and formation of receptor clusters at the postjunctional membrane. However, the underlying mechanisms are not well understood. Here we show that calpain, a calcium-dependent protease, is activated by the cholinergic stimulation and is required for induced dispersion of AChR clusters. Interestingly, the AChR-associated protein rapsyn interacted with calpain in an agrin-dependent manner, and this interaction inhibited the protease activity of calpain. Disrupting the endogenous rapsyn/calpain interaction enhanced CCh-induced dispersion of AChR clusters. Moreover, the loss of AChR clusters in agrin mutant mice was partially rescued by the inhibition of calpain via overexpressing calpastatin, an endogenous calpain inhibitor, or injecting calpeptin, a cell-permeable calpain inhibitor. These results demonstrate that calpain participates in ACh-induced dispersion of AChR clusters, and rapsyn stabilizes AChR clusters by suppressing calpain activity.
学科主题	Neurosciences & Neurology
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1758]
专题	上海神经科学研究所_神经所(总) 上海神经科学研究所_突触信号研究组
推荐引用方式 GB/T 7714	Luo, Zhen-Ge. Rapsyn interaction with calpain stabilizes AChR clusters at the neuromuscular junction[J]. NEURON,2007,55(2):247-260.
APA	Luo, Zhen-Ge.(2007).Rapsyn interaction with calpain stabilizes AChR clusters at the neuromuscular junction.NEURON,55(2),247-260.
MLA	Luo, Zhen-Ge."Rapsyn interaction with calpain stabilizes AChR clusters at the neuromuscular junction".NEURON 55.2(2007):247-260.