Reduced potassium-chloride co-transporter expression in spinal cord dorsal horn neurons contributes to inflammatory pain hypersensitivity in rats

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	Reduced potassium-chloride co-transporter expression in spinal cord dorsal horn neurons contributes to inflammatory pain hypersensitivity in rats
	Zhang, W. ; Liu, L. -Y. ; Xu, T. -L.
刊名	NEUROSCIENCE
	2008
卷号	152 期号:2 页码:502-510
关键词	chronic pain NKCC1 KCC2 disinhibition chloride homeostasis CATION-CHLORIDE COTRANSPORTER K-CL COTRANSPORTER PERIPHERAL-NERVE INJURY LAMINA-I NEURONS NEUROPATHIC PAIN GLYCINERGIC RESPONSE PYRAMIDAL NEURONS EVOKED ALLODYNIA SENSORY NEURONS ANION GRADIENT
ISSN号	0306-4522
通讯作者	Xu, TL (reprint author), Chinese Acad Sci, Inst Neurosci, 320 Yueyang Rd, Shanghai 200031, Peoples R China,tlxu@ion.ac.cn
英文摘要	Cation chloride co-transporters are important determinants for the efficacy of inhibitory neurotransmission in the spinal cord and alterations in their expression levels contribute to allodynia and hyperalgesia associated with neuropathy. However, it remains unknown whether these co-transporters contribute to chronic inflammatory pain. We investigated the expression of potassium-chloride co-transporter 2 (KCC2) and sodium-potassium-chloride co-transporter 1 (NKCC1) in the rat spinal cord after peripheral inflammation induced by complete Freund's adjuvant (CFA) injection. Our results suggest that the expression of KCC2, but not that of NKCC1, was significantly reduced in CFA-injected rats. We also found that blockade of endogenous brain-derived neurotrophic factor-tyrosine receptor kinase B pathway inhibited the inflammation-induced KCC2 downregulation. Moreover, intrathecal injection of KCC2 antisense oligodeoxynucleotides into naive rats reduced KCC2 expression in the spinal cord, leading to behavioral hypersensitivity similar to the hyperalgesia induced by peripheral inflammation. Taken together, these results indicate that peripheral inflammation induces downregulation of KCC2 in the dorsal horn of the spinal cord, which may in turn facilitate the development and/or maintenance of chronic inflammatory pain. The data also support the notion that disinhibition in the spinal cord is a general feature of inflammatory and neuropathic pain conditions, and suggest new therapeutic intervention. (C) 2008 IBRO. Published by Elsevier Ltd. All rights reserved.
学科主题	Neurosciences & Neurology
收录类别	SCI
语种	英语
公开日期	2012-07-23
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1736]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Zhang, W.,Liu, L. -Y.,Xu, T. -L.. Reduced potassium-chloride co-transporter expression in spinal cord dorsal horn neurons contributes to inflammatory pain hypersensitivity in rats[J]. NEUROSCIENCE,2008,152(2):502-510.
APA	Zhang, W.,Liu, L. -Y.,&Xu, T. -L..(2008).Reduced potassium-chloride co-transporter expression in spinal cord dorsal horn neurons contributes to inflammatory pain hypersensitivity in rats.NEUROSCIENCE,152(2),502-510.
MLA	Zhang, W.,et al."Reduced potassium-chloride co-transporter expression in spinal cord dorsal horn neurons contributes to inflammatory pain hypersensitivity in rats".NEUROSCIENCE 152.2(2008):502-510.