Development of light response and GABAergic excitation-to-inhibition switch in zebrafish retinal ganglion cells

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	Development of light response and GABAergic excitation-to-inhibition switch in zebrafish retinal ganglion cells
	Zhang, Rong-wei ; Wei, Hong-ping ; Xia, Yi-meng ; Du, Jiu-lin
刊名	JOURNAL OF PHYSIOLOGY-LONDON
	2010
卷号	588 期号:14 页码:2557-2569
关键词	VISUAL-SYSTEM DEVELOPMENT CL-COTRANSPORTER KCC2 EARLY NEURAL ACTIVITY IN-VIVO DENDRITIC GROWTH DEVELOPING RABBIT DANIO-RERIO WAVES GABA MODULATION
ISSN号	0022-3751
通讯作者	Du, JL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China,forestdu@ion.ac.cn
英文摘要	The zebrafish retina has been an important model for studying morphological development of neural circuits in vivo. However, its functional development is not yet well understood. To investigate the functional development of zebrafish retina, we developed an in vivo patch-clamp whole-cell recording technique in intact zebrafish larvae. We first examined the developmental profile of light-evoked responses (LERs) in retinal ganglion cells (RGCs) from 2 to 9 days post-fertilization (dpf). Unstable LERs were first observed at 2.5 dpf. By 4 dpf, RGCs exhibited reliable light responses. As the GABAergic system is critical for retinal development, we then performed in vivo gramicidin perforated-patch whole-cell recording to characterize the developmental change of GABAergic action in RGCs. The reversal potential of GABA-induced currents (E(GABA)) in RGCs gradually shifted from depolarized to hyperpolarized levels during 2-4 dpf and the excitation-to-inhibition (E-I) switch of GABAergic action occurred at around 2.5 dpf when RGCs became light sensitive. Meanwhile, GABAergic transmission upstream to RGCs also became inhibitory by 2.5 dpf. Furthermore, down-regulation of the K+/Cl- co-transporter (KCC2) by the morpholino oligonucleotide-based knockdown approach, which shifted RGC E(GABA) towards a more depolarized level and thus delayed the E-I switch by one day, postponed the appearance of RGC LERs by one day. In addition, RGCs exhibited correlated giant inward current (GICs) during 2.5-3.5 dpf. The period of GICs was shifted to 3-4.5 dpf by KCC2 knockdown. Taken together, the GABAergic E-I switch occurs coincidently with the emergence of light responses and GICs in zebrafish RGCs, and may contribute to the functional development of retinal circuits.
学科主题	Neurosciences & Neurology ; Physiology
收录类别	SCI
资助信息	National Basic Research Program of China[2006CB806605]; Key State Research Program of China[2006CB943802]; Shanghai government[06dj14010, 07pj14107]
语种	英语
公开日期	2012-07-13
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1586]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Zhang, Rong-wei,Wei, Hong-ping,Xia, Yi-meng,et al. Development of light response and GABAergic excitation-to-inhibition switch in zebrafish retinal ganglion cells[J]. JOURNAL OF PHYSIOLOGY-LONDON,2010,588(14):2557-2569.
APA	Zhang, Rong-wei,Wei, Hong-ping,Xia, Yi-meng,&Du, Jiu-lin.(2010).Development of light response and GABAergic excitation-to-inhibition switch in zebrafish retinal ganglion cells.JOURNAL OF PHYSIOLOGY-LONDON,588(14),2557-2569.
MLA	Zhang, Rong-wei,et al."Development of light response and GABAergic excitation-to-inhibition switch in zebrafish retinal ganglion cells".JOURNAL OF PHYSIOLOGY-LONDON 588.14(2010):2557-2569.