A Selective Filter for Cytoplasmic Transport at the Axon Initial Segment

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	A Selective Filter for Cytoplasmic Transport at the Axon Initial Segment
	Poo, Mu-ming
刊名	CELL
	2009
卷号	136 期号:6 页码:1148-1160
关键词	HIPPOCAMPAL-NEURONS ANKYRIN-G MEMBRANE-PROTEINS GLUTAMATE RECEPTORS DIFFUSION BARRIER LATERAL DIFFUSION KINESIN TRAFFICKING MECHANISMS CHANNELS
ISSN号	0092-8674
通讯作者	Duan, SM (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, State Key Lab Neurobiol, Shanghai 200031, Peoples R China,shumin@ion.ac.cn ; mpoo@berkeley.edu
英文摘要	Distinct molecules are segregated into somatodendritic and axonal compartments of polarized neurons, but mechanisms underlying the development and maintenance of such segregation remain largely unclear. In cultured hippocampal neurons, we observed an ankyrin G-and F-actin-dependent structure that emerged in the cytoplasm of the axon initial segment (AIS) within 2 days after axon/dendrite differentiation, imposing a selective filter for diffusion of macromolecules and transport of vesicular carriers into the axon. Axonal entry was allowed for KIF5-driven carriers of synaptic vesicle protein VAMP2, but not for KIF17-driven carriers of dendrite-targeting NMDA receptor subunit NR2B. Comparisons of transport rates between chimeric forms of KIF17 and KIF5B, with the motor and cargo-binding domains switched, and between KIF5 loaded with VAMP2 versus GluR2 suggest that axonal entry of vesicular carriers depends on the transport efficacy of KIF-cargo complexes. This selective AIS filtering may contribute to preferential trafficking and segregation of cellular components in polarized neurons.
学科主题	Biochemistry & Molecular Biology ; Cell Biology
收录类别	SCI
资助信息	Major State Basic Research Program of China[2006CB806600]; National Natural Science Foundation of China[30730037]
语种	英语
公开日期	2012-07-13
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/1671]
专题	上海神经科学研究所_神经所(总) 上海神经科学研究所_神经可塑性研究组
推荐引用方式 GB/T 7714	Poo, Mu-ming. A Selective Filter for Cytoplasmic Transport at the Axon Initial Segment[J]. CELL,2009,136(6):1148-1160.
APA	Poo, Mu-ming.(2009).A Selective Filter for Cytoplasmic Transport at the Axon Initial Segment.CELL,136(6),1148-1160.
MLA	Poo, Mu-ming."A Selective Filter for Cytoplasmic Transport at the Axon Initial Segment".CELL 136.6(2009):1148-1160.