Effect of Ca2+ Channel Block on Glycerol Metabolism in Dunaliella salina under Hypoosmotic and Hyperosmotic Stresses

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	Effect of Ca2+ Channel Block on Glycerol Metabolism in Dunaliella salina under Hypoosmotic and Hyperosmotic Stresses
	Chen, Hui 1,2; Chen, Shan-Li 1; Jiang, Jian-Guo 1
刊名	PLOS ONE
	2011-12-14
卷号	6 期号:12 页码:e28613
关键词	GLYCEROL-3-PHOSPHATE DEHYDROGENASE BETA-CAROTENE ALGAL CELLS CALCIUM ARABIDOPSIS BIOSYNTHESIS EXPRESSION PROTEINS SIGNALS CLONING
ISSN号	1932-6203
通讯作者	Chen, H (reprint author), S China Univ Technol, Coll Food & Bioengn, Guangzhou, Guangdong, Peoples R China.
中文摘要	The effect of Ca2+ channel blockers on cytosolic Ca2+ levels and the role of Ca2+ in glycerol metabolism of Dunaliella salina under hypoosmotic or hyperosmotic stress were investigated using the confocal laser scanning microscope (CLSM). Results showed that intracellular Ca2+ concentration increased rapidly when extracellular salinity suddenly decreased or increased, but the increase could be inhibited by pretreatment of Ca2+ channel blockers LaCl3, verapamil or ruthenium red. The changes of glycerol content and G3pdh activity in D. salina to respect to hypoosmotic or hyperosmotic stress were also inhibited in different degrees by pretreatment of Ca2+ channel blockers, indicating that the influx of Ca2+ via Ca2+ channels are required for the transduction of osmotic signal to regulate osmotic responses of D. salina to the changes of salinity. Differences of the three blockers in block effect suggested that they may act on different channels or had different action sites, including influx of Ca2+ from the extracellular space via Ca2+ channels localized in the plasma membrane or from intracellular calcium store via the mitochondrial. Other Ca2+-mediated or non-Ca2+-mediated osmotic signal pathway may exist in Dunaliella in response to hypoosmotic and hyperosmotic stresses.
英文摘要	The effect of Ca2+ channel blockers on cytosolic Ca2+ levels and the role of Ca2+ in glycerol metabolism of Dunaliella salina under hypoosmotic or hyperosmotic stress were investigated using the confocal laser scanning microscope (CLSM). Results showed that intracellular Ca2+ concentration increased rapidly when extracellular salinity suddenly decreased or increased, but the increase could be inhibited by pretreatment of Ca2+ channel blockers LaCl3, verapamil or ruthenium red. The changes of glycerol content and G3pdh activity in D. salina to respect to hypoosmotic or hyperosmotic stress were also inhibited in different degrees by pretreatment of Ca2+ channel blockers, indicating that the influx of Ca2+ via Ca2+ channels are required for the transduction of osmotic signal to regulate osmotic responses of D. salina to the changes of salinity. Differences of the three blockers in block effect suggested that they may act on different channels or had different action sites, including influx of Ca2+ from the extracellular space via Ca2+ channels localized in the plasma membrane or from intracellular calcium store via the mitochondrial. Other Ca2+-mediated or non-Ca2+-mediated osmotic signal pathway may exist in Dunaliella in response to hypoosmotic and hyperosmotic stresses.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	GLYCEROL-3-PHOSPHATE DEHYDROGENASE ; BETA-CAROTENE ; ALGAL CELLS ; CALCIUM ; ARABIDOPSIS ; BIOSYNTHESIS ; EXPRESSION ; PROTEINS ; SIGNALS ; CLONING
收录类别	SCI
资助信息	National Natural Foundation of China[30870025]
语种	英语
WOS记录号	WOS:000298369100063
公开日期	2012-09-25
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/17063]
专题	水生生物研究所_藻类生物学及应用研究中心_期刊论文
作者单位	1.S China Univ Technol, Coll Food & Bioengn, Guangzhou, Guangdong, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Chen, Hui,Chen, Shan-Li,Jiang, Jian-Guo. Effect of Ca2+ Channel Block on Glycerol Metabolism in Dunaliella salina under Hypoosmotic and Hyperosmotic Stresses[J]. PLOS ONE,2011,6(12):e28613.
APA	Chen, Hui,Chen, Shan-Li,&Jiang, Jian-Guo.(2011).Effect of Ca2+ Channel Block on Glycerol Metabolism in Dunaliella salina under Hypoosmotic and Hyperosmotic Stresses.PLOS ONE,6(12),e28613.
MLA	Chen, Hui,et al."Effect of Ca2+ Channel Block on Glycerol Metabolism in Dunaliella salina under Hypoosmotic and Hyperosmotic Stresses".PLOS ONE 6.12(2011):e28613.