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	Graphene Oxide Sheet-Mediated Silver Enhancement for Application to Electrochemical Biosensors
	Wan, Yi1,2; Wang, Yi1; Wu, Jiajia1,2; Zhag, Dun1
刊名	ANALYTICAL CHEMISTRY
	2011-02-01
卷号	83期号:3页码:648-653
ISSN号	0003-2700
通讯作者	Zhag, D (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Corros Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
中文摘要	Functionalized graphene oxide (GO) sheets coupled with a signal amplification method based on the nanomaterial-promoted reduction of silver ions for the sensitive and selective detection of bacteria. This paper aims to develop an electrochemical route combined with GO sheet-mediated Ag enhancement for biological/chemical analyte detection. A linear relationship between the stripping response and the logarithm of the bacterial concentration was obtained using an electrochemical technique for concentrations ranging from 1.8 x 10(2) to 1.8 x 10(8) cfu mL(-1), with a slope of 15.28 and a correlation coefficient of 0.995. Dot blot assay was used as a conventional immunoassay method for comparison with the electrochemical method, as well as to observe the quality of the anti-sulfite-reducing bacteria (SRB) antibody (Ab) used in the immunosensor. The GO sheet-mediated,silver enhancement holds great potential for the rapid analysis of protein, DNA, and pathogens.
英文摘要	Functionalized graphene oxide (GO) sheets coupled with a signal amplification method based on the nanomaterial-promoted reduction of silver ions for the sensitive and selective detection of bacteria. This paper aims to develop an electrochemical route combined with GO sheet-mediated Ag enhancement for biological/chemical analyte detection. A linear relationship between the stripping response and the logarithm of the bacterial concentration was obtained using an electrochemical technique for concentrations ranging from 1.8 x 10(2) to 1.8 x 10(8) cfu mL(-1), with a slope of 15.28 and a correlation coefficient of 0.995. Dot blot assay was used as a conventional immunoassay method for comparison with the electrochemical method, as well as to observe the quality of the anti-sulfite-reducing bacteria (SRB) antibody (Ab) used in the immunosensor. The GO sheet-mediated,silver enhancement holds great potential for the rapid analysis of protein, DNA, and pathogens.
学科主题	Chemistry
收录类别	SCI
原文出处	10.1021/ac103047c
语种	英语
WOS记录号	WOS:000286689600004
公开日期	2012-07-03
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/11450]
专题	海洋研究所_海洋腐蚀与防护研究发展中心
作者单位	1.Chinese Acad Sci, Shandong Prov Key Lab Corros Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Wan, Yi,Wang, Yi,Wu, Jiajia,et al. Graphene Oxide Sheet-Mediated Silver Enhancement for Application to Electrochemical Biosensors[J]. ANALYTICAL CHEMISTRY,2011,83(3):648-653.
APA	Wan, Yi,Wang, Yi,Wu, Jiajia,&Zhag, Dun.(2011).Graphene Oxide Sheet-Mediated Silver Enhancement for Application to Electrochemical Biosensors.ANALYTICAL CHEMISTRY,83(3),648-653.
MLA	Wan, Yi,et al."Graphene Oxide Sheet-Mediated Silver Enhancement for Application to Electrochemical Biosensors".ANALYTICAL CHEMISTRY 83.3(2011):648-653.

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