The Bryopsis hypnoides Plastid Genome: Multimeric Forms and Complete Nucleotide Sequence

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	The Bryopsis hypnoides Plastid Genome: Multimeric Forms and Complete Nucleotide Sequence
	Lue, Fang 1,3; Xue, Wei 2,3; Tian, Chao 1; Wang, Guangce 1; Niu, Jiangfeng 1; Pan, Guanghua 4; Hu, Songnian 3
刊名	PLOS ONE
	2011-02-14
卷号	6 期号:2 页码:e14663
ISSN号	1932-6203
中文摘要	Background: Bryopsis hypnoides Lamouroux is a siphonous green alga, and its extruded protoplasm can aggregate spontaneously in seawater and develop into mature individuals. The chloroplast of B. hypnoides is the biggest organelle in the cell and shows strong autonomy. To better understand this organelle, we sequenced and analyzed the chloroplast genome of this green alga. Principal Findings: A total of 111 functional genes, including 69 potential protein-coding genes, 5 ribosomal RNA genes, and 37 tRNA genes were identified. The genome size (153,429 bp), arrangement, and inverted-repeat (IR)-lacking structure of the B. hypnoides chloroplast DNA (cpDNA) closely resembles that of Chlorella vulgaris. Furthermore, our cytogenomic investigations using pulsed-field gel electrophoresis (PFGE) and southern blotting methods showed that the B. hypnoides cpDNA had multimeric forms, including monomer, dimer, trimer, tetramer, and even higher multimers, which is similar to the higher order organization observed previously for higher plant cpDNA. The relative amounts of the four multimeric cpDNA forms were estimated to be about 1, 1/2, 1/4, and 1/8 based on molecular hybridization analysis. Phylogenetic analyses based on a concatenated alignment of chloroplast protein sequences suggested that B. hypnoides is sister to all Chlorophyceae and this placement received moderate support. Conclusion: All of the results suggest that the autonomy of the chloroplasts of B. hypnoides has little to do with the size and gene content of the cpDNA, and the IR-lacking structure of the chloroplasts indirectly demonstrated that the multimeric molecules might result from the random cleavage and fusion of replication intermediates instead of recombinational events.
英文摘要	Background: Bryopsis hypnoides Lamouroux is a siphonous green alga, and its extruded protoplasm can aggregate spontaneously in seawater and develop into mature individuals. The chloroplast of B. hypnoides is the biggest organelle in the cell and shows strong autonomy. To better understand this organelle, we sequenced and analyzed the chloroplast genome of this green alga.
学科主题	Life Sciences & Biomedicine - Other Topics
收录类别	SCI
原文出处	10.1371/journal.pone.0014663
语种	英语
WOS记录号	WOS:000287367600003
公开日期	2012-07-03
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/11958]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Chinese Acad Sci, Beijing Genom Inst, Beijing, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 4.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin, Peoples R China
推荐引用方式 GB/T 7714	Lue, Fang,Xue, Wei,Tian, Chao,et al. The Bryopsis hypnoides Plastid Genome: Multimeric Forms and Complete Nucleotide Sequence[J]. PLOS ONE,2011,6(2):e14663.
APA	Lue, Fang.,Xue, Wei.,Tian, Chao.,Wang, Guangce.,Niu, Jiangfeng.,...&Hu, Songnian.(2011).The Bryopsis hypnoides Plastid Genome: Multimeric Forms and Complete Nucleotide Sequence.PLOS ONE,6(2),e14663.
MLA	Lue, Fang,et al."The Bryopsis hypnoides Plastid Genome: Multimeric Forms and Complete Nucleotide Sequence".PLOS ONE 6.2(2011):e14663.