DANSYLATION DERIVATIZATION ENHANCED THE DETECTION OF AMINO ACIDS BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY

	DANSYLATION DERIVATIZATION ENHANCED THE DETECTION OF AMINO ACIDS BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY
	Dai WD(戴伟东) ; Zhao CX(赵春霞) ; Kong HW(孔宏伟) ; Lu X(路鑫) ; Xu GW(许国旺)
	2011
会议名称	14th beijing conference and exhibition on instrumental analysis
会议日期	2011-10-12
会议地点	北京
页码	529-1
通讯作者	许国旺
中文摘要	high polar metabolites, containing carbohydrates, amino acids and their derivatives, and nucleosides etc, exist abundantly in plasma, urine and intracellular fluid. however, due to the poor retention on reversed-phase (rp) columns and the severe ion suppression, it is difficult to quantify these metabolites accurately by liquid chromatography mass spectrometry (lc-ms) using rp c18 columns in metabonomics studies. derivatization is a commonly used means to improve sensitivity for specific compounds when use uv or fluorescence detectors, and was applied into lc-ms analysis currently. in this study, dansyl chloride was employed to react with amino acids. 19 amino acids were selected to evaluate the performance of dansylated amino acids by lc-ms. the results (figure 1) showed that dansylation prolonged the retention times of amino acids on c18 column (changed from 1-9.5 min to 15-22 min) and enhanced the sensitivity markedly. such benefits were attributed to three major factors: 1) dansylation of amino acids improved ionization efficiency; 2) dansylation of amino acids increased the hydrophobicity of compounds; 3) elution with high organic phase leaded to the greater nebulization efficiency.
合作状况	墙报
会议主办者	中国分析测试学会
会议录	proceeding of bceia 2011
会议录出版者	待补充
会议录出版地	待补充
学科主题	分析化学
内容类型	会议论文
源URL	[http://159.226.238.44/handle/321008/116050]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Dai WD,Zhao CX,Kong HW,et al. DANSYLATION DERIVATIZATION ENHANCED THE DETECTION OF AMINO ACIDS BY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY[C]. 见:14th beijing conference and exhibition on instrumental analysis. 北京. 2011-10-12.