A bead-based approach for large-scale identification of in vitro kinase substrates

	A bead-based approach for large-scale identification of in vitro kinase substrates
	Zhang, Manyu; Han, Guanghui; Wang, Chunli; Cheng, Kai; Li, Ruibin; Liu, Hongwei; Wei, Xiaoluan; Ye, Mingliang; Zou, Hanfa
刊名	proteomics
	2011-12-01
卷号	11 期号:24 页码:4632-4637
关键词	Interaction Phosphoproteomics Phosphorylation Protein kinases Technology
ISSN号	1615-9853
通讯作者	叶明亮 ; 邹汉法
产权排序	1,1
中文摘要	a bead-based approach for large-scale identification of in vitro kinase substrates
英文摘要	deciphering the kinasesubstrate relationship is vital for the study of phosphorylation network. the use of immobilized proteins on protein chip as the library for screening of potential kinase substrates is a tried-and-tested method. however, information on phosphorylation sites is lacking and the creation of the library with proteins of whole proteome by recombinant expression is costly and difficult. in this study, a new solid-phase approach by immobilization of proteins from cell lysate onto beads as a protein library for kinase substrate screening was developed. it was found that consensus phosphorylation sites motif for kinase substrates could be accurately determined and hundreds of in vitro kinase substrates and their phosphorylation sites could be identified by using this method.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemical research methods ; biochemistry & molecular biology
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	phosphoproteome analysis ; global analysis ; phosphorylation ; ck2 ; chromatography ; enrichment ; proteomics ; sites ; liver ; cdk1
收录类别	SCI
语种	英语
WOS记录号	WOS:000297582900005
公开日期	2012-07-09
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/115568]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Manyu,Han, Guanghui,Wang, Chunli,et al. A bead-based approach for large-scale identification of in vitro kinase substrates[J]. proteomics,2011,11(24):4632-4637.
APA	Zhang, Manyu.,Han, Guanghui.,Wang, Chunli.,Cheng, Kai.,Li, Ruibin.,...&Zou, Hanfa.(2011).A bead-based approach for large-scale identification of in vitro kinase substrates.proteomics,11(24),4632-4637.
MLA	Zhang, Manyu,et al."A bead-based approach for large-scale identification of in vitro kinase substrates".proteomics 11.24(2011):4632-4637.