Analysis of low molecular weight acids by monolithic immobilized pH gradient-based capillary isoelectric focusing coupled with mass spectrometry

	Analysis of low molecular weight acids by monolithic immobilized pH gradient-based capillary isoelectric focusing coupled with mass spectrometry
	Wang, Tingting 1,2; Fekete, Agnes 3; Gaspar, Andras 3; Ma, Junfeng 1; Liang, Zhen 1; Yuan, Huiming 1; Zhang, Lihua 1; Schmitt-Kopplin, Philippe 3; Zhang, Yukui 1
刊名	journal of separation science
	2011-02-01
卷号	34 期号:4 页码:422-427
关键词	Capillary isoelectric focusing Low molecular weight acids Mass spectrometry Monolithic immobilized pH gradient
ISSN号	待补充
通讯作者	张丽华
产权排序	1,1
中文摘要	analysis of low molecular weight acids by monolithic immobilized ph gradient-based capillary isoelectric focusing coupled with mass spectrometry
英文摘要	a novel method for the separation and detection of low molecular weight (lmw) acids was developed using monolithic immobilized ph gradient-based capillary isoelectric focusing coupled with mass spectrometry. two main parameters, focusing conditions and delivery buffer conditions, which might affect separation efficiency, were optimized with the focusing time of 7 min at 350 v/cm and the delivery buffer of 50% (v/v) acetonitrile in 10 mmol/l ammonium formate (ph 3.0). under these conditions, the linear correlation between the volume of delivery solvent and the pk(a) of the model components was observed. in addition, the separation mechanism of lmw acids was proposed as well. we suppose that this method may provide a useful tool for the characterization of lmw components (e.g. natural organic matter of different origins).
学科主题	物理化学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	phase liquid-chromatography ; zone-electrophoresis ; protein-analysis ; online ; markers ; points ; device ; column
收录类别	SCI
语种	英语
WOS记录号	WOS:000287942000008
公开日期	2012-07-09
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/115316]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.German Res Ctr Environm Hlth, Helmholtz Zentrum Muenchen, Inst Ecol Chem, Neuherberg, Germany
推荐引用方式 GB/T 7714	Wang, Tingting,Fekete, Agnes,Gaspar, Andras,et al. Analysis of low molecular weight acids by monolithic immobilized pH gradient-based capillary isoelectric focusing coupled with mass spectrometry[J]. journal of separation science,2011,34(4):422-427.
APA	Wang, Tingting.,Fekete, Agnes.,Gaspar, Andras.,Ma, Junfeng.,Liang, Zhen.,...&Zhang, Yukui.(2011).Analysis of low molecular weight acids by monolithic immobilized pH gradient-based capillary isoelectric focusing coupled with mass spectrometry.journal of separation science,34(4),422-427.
MLA	Wang, Tingting,et al."Analysis of low molecular weight acids by monolithic immobilized pH gradient-based capillary isoelectric focusing coupled with mass spectrometry".journal of separation science 34.4(2011):422-427.