Size-Selective Enrichment of N-Linked Glycans Using Highly Ordered Mesoporous Carbon Material and Detection by MALDI-TOF MS

	Size-Selective Enrichment of N-Linked Glycans Using Highly Ordered Mesoporous Carbon Material and Detection by MALDI-TOF MS
	Qin, Hongqiang 1,2; Zhao, Liang 1,2; Li, Ruibin 1,2; Wu, Ren'an 1; Zou, Hanfa 1
刊名	analytical chemistry
	2011-10-15
卷号	83 期号:20 页码:7721-7728
ISSN号	0003-2700
通讯作者	邹汉法
产权排序	1,1
中文摘要	size-selective enrichment of n-linked glycans using highly ordered mesoporous carbon material and detection by maldi-tof ms
英文摘要	many diseases are characterized by the changes of either glycan structure or glycosylation site of glycoproteins. the glycan profiling can provide the overview of glycosylation in despite of the absence of the glycosylation sites, which in turn simplifies the complexity of disease diagnosis. herein, we describe a simple method to profile the n-linked glycans by maldi-tof ms with the enrichment using oxidized ordered mesoporous carbon, taking advantages of the size-exclusive effect of mesopore against proteins as well as the interaction between glycans and carbon. twenty four n-linked glycans derived from ovalbumin could be efficiently detected with high signal-to-noise (sin) ratios and sufficient peak intensities. in the analysis of complex serum samples, 32 n-linked glycans could be profiled, and 5 (4 core-fucosylated glycans) of them were distinguished from liver cancer and healthy samples.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	hepatocellular-carcinoma ; affinity-chromatography ; silica particles ; liver-cancer ; glycosylation ; oligosaccharides ; glycoproteins ; glycopeptides ; proteolysis ; extraction
收录类别	SCI
语种	英语
WOS记录号	WOS:000295817500022
公开日期	2012-07-09
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/115282]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Qin, Hongqiang,Zhao, Liang,Li, Ruibin,et al. Size-Selective Enrichment of N-Linked Glycans Using Highly Ordered Mesoporous Carbon Material and Detection by MALDI-TOF MS[J]. analytical chemistry,2011,83(20):7721-7728.
APA	Qin, Hongqiang,Zhao, Liang,Li, Ruibin,Wu, Ren'an,&Zou, Hanfa.(2011).Size-Selective Enrichment of N-Linked Glycans Using Highly Ordered Mesoporous Carbon Material and Detection by MALDI-TOF MS.analytical chemistry,83(20),7721-7728.
MLA	Qin, Hongqiang,et al."Size-Selective Enrichment of N-Linked Glycans Using Highly Ordered Mesoporous Carbon Material and Detection by MALDI-TOF MS".analytical chemistry 83.20(2011):7721-7728.