Isolation and characterization of sulfonamide-degrading bacteria Escherichia sp HS21 and Acinetobacter sp HS51

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 山东省海岸带环境工程技术研究中心

	Isolation and characterization of sulfonamide-degrading bacteria Escherichia sp HS21 and Acinetobacter sp HS51
	Zhang, Wei-Wei 1,2,3; Wen, Ying-Ying 1,2,3,4; Niu, Zong-Liang 1,2,3; Yin, Kun 1,2,3,4; Xu, Dong-Xue 5; Chen, Ling-Xin1,2,3
刊名	WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
	2012-02-01
卷号	28 期号:2 页码:447-452
关键词	Sulfonamides Escherichia sp. Acinetobacter sp. Degradation Cytotoxicity
ISSN号	0959-3993
通讯作者	Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China. lxchen@yic.ac.cn
产权排序	[Zhang, Wei-Wei; Wen, Ying-Ying; Niu, Zong-Liang; Yin, Kun; Chen, Ling-Xin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Zhang, Wei-Wei; Wen, Ying-Ying; Niu, Zong-Liang; Yin, Kun; Chen, Ling-Xin] Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang, Wei-Wei; Wen, Ying-Ying; Niu, Zong-Liang; Yin, Kun; Chen, Ling-Xin] Chinese Acad Sci, Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China; [Wen, Ying-Ying; Yin, Kun] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Xu, Dong-Xue] China Agr Univ Yantai, Dept Aquaculture, Yantai 264670, Peoples R China
中文摘要	With the intensive application of sulfonamides in aquaculture and animal husbandry and the increase of sulfonamides discharged into the environments, there is an increasing need to find a way to remediate sulfonamide-contaminated environments. Two bacterial strains capable of degrading sulfonamides, HS21 and HS51, were isolated from marine environments. HS21 and HS51 were identified as members of Escherichia sp. and Acinetobacter sp., respectively, based on 16S rRNA gene sequencing. Degradation of each sulfonamide by Escherichia sp. HS21 and Acinetobacter sp. HS51 was characterized using capillary electrophoresis. About 66 or 72% of sulfapyridine and 45 or 67% of sulfathiazole contained in the media was degraded by Escherichia sp. HS21 or Acinetobacter sp. HS51, respectively, after incubation for 2 days. The supernatant from culture of Escherichia sp. HS21 or Acinetobacter sp. HS51 grown in sulfapyridine or sulfathiazole contained media had much attenuated cytotoxicity against HeLa cells. These results suggest that Escherichia sp. HS21 and Acinetobacter sp. HS51 are new bacterial resources for biodegrading sulfonamides and indicate the potential of isolated strains for the bioremediation of sulfonamide-polluted environments.
英文摘要	With the intensive application of sulfonamides in aquaculture and animal husbandry and the increase of sulfonamides discharged into the environments, there is an increasing need to find a way to remediate sulfonamide-contaminated environments. Two bacterial strains capable of degrading sulfonamides, HS21 and HS51, were isolated from marine environments. HS21 and HS51 were identified as members of Escherichia sp. and Acinetobacter sp., respectively, based on 16S rRNA gene sequencing. Degradation of each sulfonamide by Escherichia sp. HS21 and Acinetobacter sp. HS51 was characterized using capillary electrophoresis. About 66 or 72% of sulfapyridine and 45 or 67% of sulfathiazole contained in the media was degraded by Escherichia sp. HS21 or Acinetobacter sp. HS51, respectively, after incubation for 2 days. The supernatant from culture of Escherichia sp. HS21 or Acinetobacter sp. HS51 grown in sulfapyridine or sulfathiazole contained media had much attenuated cytotoxicity against HeLa cells. These results suggest that Escherichia sp. HS21 and Acinetobacter sp. HS51 are new bacterial resources for biodegrading sulfonamides and indicate the potential of isolated strains for the bioremediation of sulfonamide-polluted environments.
学科主题	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	TANDEM MASS-SPECTROMETRY ; WASTE-WATER CONTAMINANTS ; AQUATIC ENVIRONMENT ; NATIONAL RECONNAISSANCE ; HUMAN PHARMACEUTICALS ; CARBONIC-ANHYDRASE ; UNITED-STATES ; P-TSA ; O-TSA ; ANTIBIOTICS
收录类别	SCI
资助信息	Chinese Academy of Sciences[KZCX2-EW-206]; National Natural Science Foundation of China (NSFC)[20975089]; Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences[Kf201012]; Department of Science and Technology of Yantai City of China[2010235]
原文出处	http://www.springerlink.com/content/0959-3993/
语种	英语
WOS记录号	WOS:000300014300005
公开日期	2012-06-13
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/5506]
专题	烟台海岸带研究所_山东省海岸带环境工程技术研究中心
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 5.China Agr Univ Yantai, Dept Aquaculture, Yantai 264670, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Wei-Wei,Wen, Ying-Ying,Niu, Zong-Liang,et al. Isolation and characterization of sulfonamide-degrading bacteria Escherichia sp HS21 and Acinetobacter sp HS51[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2012,28(2):447-452.
APA	Zhang, Wei-Wei,Wen, Ying-Ying,Niu, Zong-Liang,Yin, Kun,Xu, Dong-Xue,&Chen, Ling-Xin.(2012).Isolation and characterization of sulfonamide-degrading bacteria Escherichia sp HS21 and Acinetobacter sp HS51.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,28(2),447-452.
MLA	Zhang, Wei-Wei,et al."Isolation and characterization of sulfonamide-degrading bacteria Escherichia sp HS21 and Acinetobacter sp HS51".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 28.2(2012):447-452.