Proteomics of desiccation tolerance during development and germination of maize embryos

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	Proteomics of desiccation tolerance during development and germination of maize embryos
	Huang, Hui 1,2; Moller, Ian Max 3; Song, Song-Quan 1
刊名	JOURNAL OF PROTEOMICS
	2012-02-02
卷号	75 期号:4 页码:1247-1262
关键词	Desiccation tolerance Development Germination Maize embryos Proteomic analysis
ISSN号	1874-3919 ; 1874-3919
通讯作者	Song, SQ (reprint author), Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China, sqsong@ibcas.ac.cn ; Song, SQ (reprint author), Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China, sqsong@ibcas.ac.cn
英文摘要	Maize seeds were used to identify the key embryo proteins involved in desiccation tolerance during development and germination. Immature maize embryos (28N) during development and mature embryos imbibed for 72 h (72HN) are desiccation sensitive. Mature maize embryos (52N) during development are desiccation tolerant Thiobarbituric acid reactive substance and hydrogen peroxide contents decreased and increased with acquisition and loss of desiccation tolerance, respectively. A total of 111 protein spots changed significantly (1.5 fold increase/decrease) in desiccation-tolerant and -sensitive embryos before (28N, 52N and 72HN) and after (280, 520 and 72HD) dehydration. Nine pre-dominantly proteins, 17.4 kDa Class I heat shock protein 3, late embiyogenesis abundant protein EMB564, outer membrane protein, globulin 2, TPA:putative cystatin, NBS-LFtR resistance-like protein RGC456, stress responsive protein, major allergen Bet v 1.01C and proteasome subunit alpha type 1, accumulated during embryo maturation, decreased during germination and increased in desiccation-tolerant embryos during desiccation. Two proteins, Rhd6-like 2 and low-molecular-weight heat shock protein precursor, showed the inverse pattern. We infer that these eleven proteins are involved in seed desiccation tolerance. We conclude that desiccation-tolerant embryos make more economical use of their resources to accumulate protective molecules and antioxidant systems to deal with maturation drying and desiccation treatment. (C) 2011 Elsevier B.V. All rights reserved.
学科主题	Biochemical Research Methods ; Biochemical Research Methods
类目[WOS]	Biochemical Research Methods
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	EMBRYOGENESIS-ABUNDANT PROTEIN ; HEAT-SHOCK PROTEINS ; RICE ORYZA-SATIVA ; ARABIDOPSIS-THALIANA ; HYDROGEN-PEROXIDE ; SEED-GERMINATION ; ACTIVE OXYGEN ; BEAN-SEEDS ; STRESS ; PLANTS
收录类别	SCI
资助信息	National Natural Sciences Foundation of China[30870223]; Chinese Academy of Sciences[KZCX2-YW-414]; National Natural Sciences Foundation of China[30870223]; Chinese Academy of Sciences[KZCX2-YW-414]
语种	英语
WOS记录号	WOS:000300533600015
公开日期	2012-06-07 ; 2012-06-07
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/10527]
专题	昆明植物研究所_中国西南野生生物种质资源库
作者单位	1.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China 3.Aarhus Univ, Dept Mol Biol & Biotechnol, DK-4200 Slagelse, Denmark
推荐引用方式 GB/T 7714	Huang, Hui,Moller, Ian Max,Song, Song-Quan. Proteomics of desiccation tolerance during development and germination of maize embryos[J]. JOURNAL OF PROTEOMICS,2012,75(4):1247-1262.
APA	Huang, Hui,Moller, Ian Max,&Song, Song-Quan.(2012).Proteomics of desiccation tolerance during development and germination of maize embryos.JOURNAL OF PROTEOMICS,75(4),1247-1262.
MLA	Huang, Hui,et al."Proteomics of desiccation tolerance during development and germination of maize embryos".JOURNAL OF PROTEOMICS 75.4(2012):1247-1262.