SAW: A Method to Identify Splicing Events from RNA-Seq Data based on Splicing Fingerprints | |
Kang Ning ; Damian Fermin | |
刊名 | PLoS ONE |
2010 | |
期号 | 3 |
关键词 | SAW: A Method to Identify Splicing Events from RNA-Seq Data based on Splicing Fingerprints |
中文摘要 | Splicing event identification is one of the most important issues in the comprehensive analysis of transcription profile. Recent development of next-generation sequencing technology has generated an extensive profile of alternative splicing. However, while many of these splicing events are between exons that are relatively close on genome sequences, reads generated by RNA-Seq are not limited to alternative splicing between close exons but occur in virtually all splicing events. In this work, a novel method, SAW, was proposed for the identification of all splicing events based on short reads from RNASeq. It was observed that short reads not in known gene models are actually absent words from known gene sequences. An efficient method to filter and cluster these short reads by fingerprint fragments of splicing events without aligning short reads to genome sequences was developed. Additionally, the possible splicing sites were also determined without alignment against genome sequences. A consensus sequence was then generated for each short read cluster, which was then aligned to the genome sequences. Results demonstrated that this method could identify more than 90% of the known splicing events with a very low false discovery rate, as well as accurately identify, a number of novel splicing events between distant exons. |
英文摘要 | Splicing event identification is one of the most important issues in the comprehensive analysis of transcription profile. Recent development of next-generation sequencing technology has generated an extensive profile of alternative splicing. However, while many of these splicing events are between exons that are relatively close on genome sequences, reads generated by RNA-Seq are not limited to alternative splicing between close exons but occur in virtually all splicing events. In this work, a novel method, SAW, was proposed for the identification of all splicing events based on short reads from RNASeq. It was observed that short reads not in known gene models are actually absent words from known gene sequences. An efficient method to filter and cluster these short reads by fingerprint fragments of splicing events without aligning short reads to genome sequences was developed. Additionally, the possible splicing sites were also determined without alignment against genome sequences. A consensus sequence was then generated for each short read cluster, which was then aligned to the genome sequences. Results demonstrated that this method could identify more than 90% of the known splicing events with a very low false discovery rate, as well as accurately identify, a number of novel splicing events between distant exons. |
学科主题 | 功能基因组 |
公开日期 | 2012-06-01 |
内容类型 | 期刊论文 |
源URL | [http://ir.qibebt.ac.cn:8080/handle/337004/974] |
专题 | 青岛生物能源与过程研究所_单细胞中心 |
推荐引用方式 GB/T 7714 | Kang Ning,Damian Fermin. SAW: A Method to Identify Splicing Events from RNA-Seq Data based on Splicing Fingerprints[J]. PLoS ONE,2010(3). |
APA | Kang Ning,&Damian Fermin.(2010).SAW: A Method to Identify Splicing Events from RNA-Seq Data based on Splicing Fingerprints.PLoS ONE(3). |
MLA | Kang Ning,et al."SAW: A Method to Identify Splicing Events from RNA-Seq Data based on Splicing Fingerprints".PLoS ONE .3(2010). |
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