Optimization using central composite design for antihistamines separation by nonaqueous capillary electrophoresis with electrochemical and electrochemiluminescence detections

	Optimization using central composite design for antihistamines separation by nonaqueous capillary electrophoresis with electrochemical and electrochemiluminescence detections
	Li X ; Xu XY ; Albano DR ; You TY
刊名	analyst
	2011
卷号	136 期号:24 页码:5294-5301
关键词	MASS-SPECTROMETRY LIQUID-CHROMATOGRAPHY HUMAN PLASMA ZONE-ELECTROPHORESIS FACTORIAL DESIGN DIPHENHYDRAMINE CHLORPHENIRAMINE HYDROCHLORIDE DEXTROMETHORPHAN PSEUDOEPHEDRINE
ISSN号	0003-2654
通讯作者	you ty
中文摘要	the first detailed examination of non-aqueous capillary electrophoresis with electrochemical and electrochemiluminescence detections (nace-ec/ecl), separation parameters and their interactions via central composite design was presented. this concept was demonstrated by examining the optimization separation conditions of seven antihistamines (chlorpheniramine, cyproheptadine, diphenhydramine, doxylamine, methapyrilene, terfenadine, and triprolidine) by nace-ec/ecl. to evaluate the nace separation quality, the chromatography resolution statistic function (crs(-1) function) with regard to the resolution and migration time was established as the response variable. the influences of three experimental variables (buffer apparent ph value (ph), buffer (tbap) concentration, and separation voltage) on the response were investigated. a set of optimal conditions was obtained from central composite design: 9.2 mm tbap in acn (ph 4.0) and voltage (12.7 kv), and under these optimum conditions, the seven antihistamines could be well separated in less than 10 min. the obtained electropherograms indicated that the dual ec/ecl detection system was indispensable since the six antihistamines (except for triprolidine) displayed both ec and ecl response, whereas triprolidine only displayed the ec response. this work is instructive for investigators in simplifying the nace-ec/ecl development procedures for multi-component analysis.
语种	英语
WOS记录号	WOS:000297223000029
公开日期	2012-06-11
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/44901]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Li X,Xu XY,Albano DR,et al. Optimization using central composite design for antihistamines separation by nonaqueous capillary electrophoresis with electrochemical and electrochemiluminescence detections[J]. analyst,2011,136(24):5294-5301.
APA	Li X,Xu XY,Albano DR,&You TY.(2011).Optimization using central composite design for antihistamines separation by nonaqueous capillary electrophoresis with electrochemical and electrochemiluminescence detections.analyst,136(24),5294-5301.
MLA	Li X,et al."Optimization using central composite design for antihistamines separation by nonaqueous capillary electrophoresis with electrochemical and electrochemiluminescence detections".analyst 136.24(2011):5294-5301.