CHARACTERIZATION OF A DRE-BINDING TRANSCRIPTION FACTOR FROM ASPARAGUS (ASPARAGUS OFFICINALIS L.) AND ITS OVEREXPRESSION IN ARABIDOPSIS RESULTING IN SALT- AND DROUGHT-RESISTANT TRANSGENIC PLANTS

CORC > 地理科学与资源研究所 > 中国科学院地理科学与资源研究所 > 自然资源与环境安全研究部 > 环境修复与资源工程研究室 > SCI/SSCI期刊论文

	CHARACTERIZATION OF A DRE-BINDING TRANSCRIPTION FACTOR FROM ASPARAGUS (ASPARAGUS OFFICINALIS L.) AND ITS OVEREXPRESSION IN ARABIDOPSIS RESULTING IN SALT- AND DROUGHT-RESISTANT TRANSGENIC PLANTS
	Jiang D.; Zhuang D. F.
	2010
关键词	AP2/EREBP domain Asparagus officinalis DRE-binding protein stress tolerance transcription factor yeast one-hybrid responsive gene-expression zea-mays-l low-temperature functional-analysis freezing-tolerance ap2 domain erf/ap2 domain abiotic stress high-salinity cold
英文摘要	A new full-length cDNA encoding an AP2/EREBP domain-containing transcription factor named AoDREB was isolated from Asparagus officinalis L. using the RACE-PCR method. It is a homolog to the dehydration-responsive element binding protein (DREB) and classified to the A6 subgroup of the DREB subfamily. Using the yeast one-hybrid system, we conducted a DRE binding assay and demonstrated that AoDREB can bind the DRE element specifically. A transcriptional activity assay showed that AoDREB is a transcription factor capable of activating expression of the reporter gene in yeast. RT-PCR analysis revealed that expression of the AoDREB gene is induced under 20% PEG and high salinity stress, whereas no obvious response to low temperature was observed. Overexpression of AoDREB in transgenic Arabidopsis caused no growth retardation and induced stronger expression of the target genes, including RD29A and COR15A, than in wild type, resulting in increased tolerance of transgenic Arabidopsis to drought and high salinity. These results suggest that introduction of an exogenous AoDREB into plants is an effective way to improve plant drought and salinity tolerance.
出处	International Journal of Plant Sciences
卷	171
期	1
页	12-23
收录类别	SCI
语种	英语
ISSN号	1058-5893
内容类型	SCI/SSCI论文
源URL	[http://ir.igsnrr.ac.cn/handle/311030/23406]
专题	资源利用与环境修复重点实验室_资源地理与国土资源研究室_SCI/SSCI期刊论文中国科学院地理科学与资源研究所
推荐引用方式 GB/T 7714	Jiang D.,Zhuang D. F.. CHARACTERIZATION OF A DRE-BINDING TRANSCRIPTION FACTOR FROM ASPARAGUS (ASPARAGUS OFFICINALIS L.) AND ITS OVEREXPRESSION IN ARABIDOPSIS RESULTING IN SALT- AND DROUGHT-RESISTANT TRANSGENIC PLANTS. 2010.