Chitosan cushioned phospholipid membrane and its application in imaging ellipsometry based-biosensor | |
Zhang YB; Chen YY(陈艳艳); Jin G(靳刚) | |
刊名 | Applied Surface Science |
2011 | |
通讯作者邮箱 | ybzhang2007@sinano.ac.cn; yychen2006@sinano.ac.cn; gajin@imech.ac.cn |
卷号 | 257期号:22页码:9407-9413 |
关键词 | Phospholipid Membrane Chitosan Imaging Ellipsometry Biosensor Afm Self-Assembled Monolayers Supported Lipid-Bilayers Nonspecific Protein Adsorption Surface-Plasmon Resonance Molecular-Conformation Cell-Membrane Resist Cholesterol Adhesion Microarrays |
ISSN号 | 0169-4332 |
通讯作者 | Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Jiangsu, Peoples R China |
产权排序 | [Zhang, Yibang; Chen, Yanyan; Jin, Gang] Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215125, Jiangsu, Peoples R China; [Zhang, Yibang; Jin, Gang] Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China; [Zhang, Yibang] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Jin, Gang] Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China |
合作状况 | 国内 |
中文摘要 | Chitosan cushion can support the air stability of phospholipid membrane, but the problem of serum solubility of phospholipid membrane prevents it from use in serum detection applications. Poly (ethylene glycol) (PEG) shielding promises both stability and non-specific adsorption resistance for phospholipid membrane. An air stable phospholipid membrane microarray has been successfully fabricated on chitosan modified silicon wafer. We have demonstrated the potential application of PEGylated phospholipid membrane in imaging ellipsometry-based protein biosensor. Because of the strong resistance against non-specific adsorption of serum, antigens are immobilized onto the membrane surface through chemical activation and further bind their antibodies without using blocking agent. Taking advantage of the multiple and parallel reaction capabilities of microfluidic reactor system, we have assayed the binding by varying both the density of antigen on the membrane surface and the concentration of antibody in solution. (C) 2011 Elsevier B. V. All rights reserved. |
学科主题 | Chemistry; Materials Science; Physics |
分类号 | 一类 |
类目[WOS] | Chemistry, Physical ; Materials Science, Coatings & Films ; Physics, Applied ; Physics, Condensed Matter |
研究领域[WOS] | Chemistry ; Materials Science ; Physics |
关键词[WOS] | SELF-ASSEMBLED MONOLAYERS ; SUPPORTED LIPID-BILAYERS ; NONSPECIFIC PROTEIN ADSORPTION ; SURFACE-PLASMON RESONANCE ; MOLECULAR-CONFORMATION ; CELL-MEMBRANE ; RESIST ; CHOLESTEROL ; ADHESION ; MICROARRAYS |
收录类别 | SCI ; EI |
资助信息 | The authors would like to gratefully acknowledge financial supports from Ministry of Science and Technology of China (2008AA02Z419, 2009CB320302), Natural Science Foundation (10804083, 20845003) of China, and Key Laboratory of Microgravity, Institute of Mechanics, CAS. |
原文出处 | http://dx.doi.org/10.1016/j.apsusc.2011.06.019 |
语种 | 英语 |
WOS记录号 | WOS:000293590300031 |
公开日期 | 2012-04-01 |
内容类型 | 期刊论文 |
源URL | [http://dspace.imech.ac.cn/handle/311007/45076] |
专题 | 力学研究所_国家微重力实验室 |
推荐引用方式 GB/T 7714 | Zhang YB,Chen YY,Jin G. Chitosan cushioned phospholipid membrane and its application in imaging ellipsometry based-biosensor[J]. Applied Surface Science,2011,257(22):9407-9413. |
APA | Zhang YB,陈艳艳,&靳刚.(2011).Chitosan cushioned phospholipid membrane and its application in imaging ellipsometry based-biosensor.Applied Surface Science,257(22),9407-9413. |
MLA | Zhang YB,et al."Chitosan cushioned phospholipid membrane and its application in imaging ellipsometry based-biosensor".Applied Surface Science 257.22(2011):9407-9413. |
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