The promotion of neurite sprouting and outgrowth of mouse hippocampal cells in culture by graphene substrates

	The promotion of neurite sprouting and outgrowth of mouse hippocampal cells in culture by graphene substrates
	Li N(李宁); Zhang XM; Song Q; Su RG(苏瑞巩); Zhang Q; Kong T; Liu LW; Jin G(靳刚); Tang ML; Cheng GS
刊名	Biomaterials
	2011
通讯作者邮箱	mltang2010@sinano.ac.cn; gscheng2006@sinano.ac.cn
卷号	32 期号:35 页码:9374-9382
关键词	Neuron-Favorable Cvd Graphene Gap-43 Neurite Sprouting & Outgrowth Hippocampus Few-Layer Graphene Carbon Nanotubes Neuronal Growth Large-Area Oxide Interfaces Films Cytotoxicity Delivery Systems
ISSN号	0142-9612
通讯作者	Tang, ML (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, 398 Ruoshui Rd, Suzhou Ind Pk 215123, Peoples R China
产权排序	[Li, Ning; Zhang, Xuemin; Song, Qin; Su, Ruigong; Zhang, Qi; Kong, Tao; Liu, Liwei; Tang, Mingliang; Cheng, Guosheng] Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou Ind Pk 215123, Peoples R China; [Jin, Gang] Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China; [Li, Ning; Su, Ruigong; Liu, Liwei; Jin, Gang; Tang, Mingliang; Cheng, Guosheng] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Zhang, Xuemin] Changchun Univ Sci & Technol, Changchun 130022, Jilin, Peoples R China
合作状况	国内
中文摘要	Graphene has been demonstrated in many biomedical applications and its potentials for neural interfacing. Emerging concerns on graphene, as a biomedical material, are its biocompatibility and how biologically targeted tissue/cells respond to it. Relatively few studies attempted to address the interactions of graphene or its derivatives with the tissues/cells, while very few reports on neural system. In this study, we tried to explore how neurites, one of the key structures for neural functions, are affected by graphene during the development until maturation in a mouse hippocampal culture model. The results reveal that graphene substrates exhibited excellent biocompatibility, as cell viability and morphology were not affected. Meanwhile, neurite numbers and average neurite length on graphene were significantly enhanced during 2-7 days after cell seeding compared with tissue culture polystyrene (TCPS) substrates. Especially on Day 2 of the neural development period, graphene substrates efficiently promoted neurite sprouting and outgrowth to the maximal extent. Additionally, expression of growth-associate protein-43 (GAP-43) was examined in both graphene and TCPS groups. Western blot analysis showed that GAP-43 expression was greatly enhanced in graphene group compared to TCPS group, which might result in the boost of neurite sprouting and outgrowth. This study suggests the potential of graphene as a material for neural interfacing and provides insight into the future biomedical applications of graphene. (C) 2011 Elsevier Ltd. All rights reserved.
学科主题	Engineering; Materials Science
分类号	一类
类目[WOS]	Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]	Engineering ; Materials Science
关键词[WOS]	FEW-LAYER GRAPHENE ; CARBON NANOTUBES ; NEURONAL GROWTH ; LARGE-AREA ; OXIDE ; INTERFACES ; FILMS ; CYTOTOXICITY ; DELIVERY ; SYSTEMS
收录类别	SCI ; EI
资助信息	This work was partially funded by National Basic Research Program of China under award numbers of 2011CB965004, Fund for Presidential Award Winners of Chinese Academy of Sciences (Y1BFS11001) and "100-Talents Program" of Chinese Academy of Sciences. This work was made possible by extraordinarily professional services by Nano-Fabrication & Nano-Characterization Platforms with Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences. We thank Mr. Weiwei Li for the help of graphene synthesis.
原文出处	http://dx.doi.org/10.1016/j.biomaterials.2011.08.065
语种	英语
WOS记录号	WOS:000296684200023
公开日期	2012-04-01
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/45065]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Li N,Zhang XM,Song Q,et al. The promotion of neurite sprouting and outgrowth of mouse hippocampal cells in culture by graphene substrates[J]. Biomaterials,2011,32(35):9374-9382.
APA	李宁.,Zhang XM.,Song Q.,苏瑞巩.,Zhang Q.,...&Cheng GS.(2011).The promotion of neurite sprouting and outgrowth of mouse hippocampal cells in culture by graphene substrates.Biomaterials,32(35),9374-9382.
MLA	李宁,et al."The promotion of neurite sprouting and outgrowth of mouse hippocampal cells in culture by graphene substrates".Biomaterials 32.35(2011):9374-9382.