Molecular cloning and sequence analysis of the duck enteritis virus UL5 gene

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	Molecular cloning and sequence analysis of the duck enteritis virus UL5 gene
	Pana, HQ ; Cao, RB ; Liu, L ; Niu, MF ; Zhou, B ; Chen, PY ; Hua, JC
刊名	VIRUS RESEARCH
	2008
卷号	136 期号:1-2 页码:152-156
关键词	duck enteritis virus UL5 gene gene character phylogenetic analysis
ISSN号	0168-1702
通讯作者	Cao, RB, Nanjing Agr Univ, Key Lab Anim Dis Diag & Immunol, Coll Vet Med, Nanjing 210095, Peoples R China
中文摘要	Duck enteritis virus (DEV) is a herpesvirus that causes an acute, contagious, and fatal disease. In the present article, the DEV UL5 gene was cloned and sequenced from a vaccine virus. According to the consensus sequence of herpesvirus UL5 and UL3 gene degenerate oligonucleotide primers were designed and were used in the polymerase chain reaction (PCR) to amplify DNA products with 4577 bp in size. DNA sequence analysis revealed a 2568 bp open reading frame (ORF) encoding a 855 amino acid polypeptide homologous to herpesvirus UL5 proteins. The DEV UL5 gene has a base composition of 769 adenine (29.95%), 556 cytosine (21.65%),533 guanine (20.76%) and 710 thymine (27.65%). Sequence comparison revealed that the nucleotide sequence of the DEV UL5 gene was highly similar to other alphaherpesviruses. Phylogenetic tree analysis showed that the fifteen herpesviruses viruses analyzed fell into four large groups, and the duck enteritis virus itself branched and was most closely related to meleagrid herpesvirus 1, gallid herpesvirus 2 and gallid herpesvirus 3 subtrees. (c) 2008 Elsevier B.V. All rights reserved.
学科主题	Virology
收录类别	SCI
语种	英语
WOS记录号	WOS:000258009100020
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/54407]
专题	沈阳应用生态研究所_沈阳应用生态研究所_期刊论文
推荐引用方式 GB/T 7714	Pana, HQ,Cao, RB,Liu, L,et al. Molecular cloning and sequence analysis of the duck enteritis virus UL5 gene[J]. VIRUS RESEARCH,2008,136(1-2):152-156.
APA	Pana, HQ.,Cao, RB.,Liu, L.,Niu, MF.,Zhou, B.,...&Hua, JC.(2008).Molecular cloning and sequence analysis of the duck enteritis virus UL5 gene.VIRUS RESEARCH,136(1-2),152-156.
MLA	Pana, HQ,et al."Molecular cloning and sequence analysis of the duck enteritis virus UL5 gene".VIRUS RESEARCH 136.1-2(2008):152-156.