DNA mismatch repair related gene expression as potential biomarkers to assess cadmium exposure in Arabidopsis seedlings

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	DNA mismatch repair related gene expression as potential biomarkers to assess cadmium exposure in Arabidopsis seedlings
	Liu, W ; Zhou, QX ; Li, PJ ; Gao, HR ; Han, YP ; Li, XJ ; Yang, YS ; Li, YZ
刊名	JOURNAL OF HAZARDOUS MATERIALS
	2009
卷号	167 期号:1-3 页码:1007-1013
关键词	Cadmium atMLH1 and atMSH2 3 6 7 genes atPCNA1 2 genes Arabidopsis thaliana
ISSN号	0304-3894
通讯作者	Zhou, QX, Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China
中文摘要	In the current study, Arabidopsis seedlings were hydroponically grown on MS media containing cadmium (Cd)of 0-2.0 mg L-1 for 60 h of treatment. Gene expression profiles were used to relate exposure to Cd with some altered biological responses and/or specific growth effects. RT-PCR analysis was used to quantitate mRNA expression for seven genes known to be involved in DNA mismatch repair (MMR) system and cell division. Results indicated that Cd concentrations of 0.25-2.0 mg L-1 cause increased total soluble protein levels in shoots of Arabidopsis seedlings in an inverted U-shaped dose-response manner. Exposure to 0.25 and 0.5 mg L-1 of Cd dramatically induced expression of four genes (i.e. proliferating cell nuclear antigen 2 (atPCNA 2), MutL1 homolog (atMLH1), MutS 2 homolog (atMSH2) and atMSH3) and five genes (i.e. atPCNA1,2, atMLH1 and atMSH2,7), respectively, in shoots of Arabidopsis seedlings; Exposure to 1.0 mg L-1 of Cd significantly elevated expression of only two genes (atMSH6,7), but caused prominent inhibition in expression of three genes (atPCNA2, atMLH1 and atMSH3) in shoots of Arabidopsis seedlings. The expression alterations of the above genes were independent of any biological effects such as survival, fresh weight and chlorophyll level of shoots. However, shoots of Arabidopsis seedlings exposed to 2.0 mg L-1 of Cd exhibited statistically prominent repression in expression of these seven genes, and showed incipient reduction of fresh weight and chlorophyll level. This research provides data concerning sensitivity of expression profiles of atMLH1, atMSH2,3,6,7 and atPCNA1,2 genes in Arabidopsis seedlings to Cd exposure, as well as the potential use of these gene expression patterns as representative molecular biomarkers indicative of Cd exposure and related biological effects. (C) 2009 Elsevier B.V. All rights reserved.
学科主题	Engineering ; Environmental; Engineering ; Civil; Environmental Sciences
收录类别	SCI
资助信息	Chinese Academy of Sciences [kzcx1-yw-06-03]; 973 Project [2004CB418506]; National Natural Science Foundation of PR China [20377043]
语种	英语
WOS记录号	WOS:000267267000146
公开日期	2011-09-23
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/54115]
专题	沈阳应用生态研究所_沈阳应用生态研究所_期刊论文
推荐引用方式 GB/T 7714	Liu, W,Zhou, QX,Li, PJ,et al. DNA mismatch repair related gene expression as potential biomarkers to assess cadmium exposure in Arabidopsis seedlings[J]. JOURNAL OF HAZARDOUS MATERIALS,2009,167(1-3):1007-1013.
APA	Liu, W.,Zhou, QX.,Li, PJ.,Gao, HR.,Han, YP.,...&Li, YZ.(2009).DNA mismatch repair related gene expression as potential biomarkers to assess cadmium exposure in Arabidopsis seedlings.JOURNAL OF HAZARDOUS MATERIALS,167(1-3),1007-1013.
MLA	Liu, W,et al."DNA mismatch repair related gene expression as potential biomarkers to assess cadmium exposure in Arabidopsis seedlings".JOURNAL OF HAZARDOUS MATERIALS 167.1-3(2009):1007-1013.