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	Delivery system for DNAzymes using arginine-modified hydroxyapatite nanoparticles for therapeutic application in a nasopharyngeal carcinoma model
	Chen, Yan; Yang, Lifang*; Huang, Suping; Li, Zhi; Zhang, Lu; He, Jiang; Xu, Zhijie; Liu, Liyu; Cao, Ya; Sun, Lunquan
刊名	International Journal of Nanomedicine
	2013
卷号	8期号:1页码:3107-3118
关键词	DNAzymes are synthetic single-stranded catalytic nucleic acids that bind and cleave target mRNA in a sequence-specific manner and have been explored for genotherapeutics. One bottleneck restricting their application is the lack of an efficient delivery system. As an inorganic nanomaterial with potentially wide application nanohydroxyapatite particles (nHAP) have attracted increasing attention as new candidates for nonviral vectors. In this study we developed an nHAP-based delivery system and explored its cellular uptake mechanisms intracellular localization and biological effects. Absorption of arginine-modified nanohydroxyapatite particles (Arg-nHAP) and DZ1 (latent membrane protein 1 [LMP1]-targeted) reached nearly 100% efficiency under in vitro conditions. Using specific inhibitors cellular uptake of the Arg-nHAP/DZ1 complex was shown to be mediated by the energy-dependent endocytosis pathway. Further effective intracellular delivery and nuclear localization of the complex was confirmed by confocal microscopy. Biologically the complex successfully downregulated the expression of LMP1 in nasopharyngeal carcinoma cells. In a mouse tumor xenograft model the complex was shown to be delivered efficiently to tumor tissue downregulating expression of LMP1 and suppressing tumor growth. These results suggest that Arg-nHAP may be an efficient vector for nucleic acid-based drugs with potential clinical application.
ISSN号	1178-2013
DOI	10.2147/IJN.S48321
URL标识	查看原文
WOS记录号	WOS:000323096700001
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/3325181
专题	中南大学
作者单位	1.[Yang, Lifang 2.Zhang, Lu 3.Li, Zhi 4.Chen, Yan 5.He, Jiang 6.Sun, Lunquan] Cent S Univ, Xiangya Hosp, Ctr Mol Med, Changsha 410008, Hunan, Peoples R China.
推荐引用方式 GB/T 7714	Chen, Yan,Yang, Lifang*,Huang, Suping,et al. Delivery system for DNAzymes using arginine-modified hydroxyapatite nanoparticles for therapeutic application in a nasopharyngeal carcinoma model[J]. International Journal of Nanomedicine,2013,8(1):3107-3118.
APA	Chen, Yan.,Yang, Lifang*.,Huang, Suping.,Li, Zhi.,Zhang, Lu.,...&Sun, Lunquan.(2013).Delivery system for DNAzymes using arginine-modified hydroxyapatite nanoparticles for therapeutic application in a nasopharyngeal carcinoma model.International Journal of Nanomedicine,8(1),3107-3118.
MLA	Chen, Yan,et al."Delivery system for DNAzymes using arginine-modified hydroxyapatite nanoparticles for therapeutic application in a nasopharyngeal carcinoma model".International Journal of Nanomedicine 8.1(2013):3107-3118.

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