Effects on Expression of Hepatic Phase-1 and Phase-2 Metabolism and Transporter Genes by Swertia mussotii Extract, A Hepatoprotective Herb from Tibetan Traditional Medicine

	Effects on Expression of Hepatic Phase-1 and Phase-2 Metabolism and Transporter Genes by Swertia mussotii Extract, A Hepatoprotective Herb from Tibetan Traditional Medicine
	Yang, Hongxia; Xiao, Yuancan; Li, Cen; Gao, Tingting; Wei, Lixin; Du, Yuzhi
刊名	INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY
	2019
卷号	21 期号:5
关键词	Tibetan traditional medicine Swertia mussotii Cytochrome P450 Hepatic transporters
英文摘要	To study the effects of Swertia mussotii extract on the expression of drug processing genes, including Phase-1 (cytochrome P450 enzyme genes), Phase-2 (glucuronidation and sulfation genes) and Phase-3 (drug transporters) in mouse liver. Mice were orally administered S. mussotii extract at the dose of 0.1, 0.3, 1.0, 3.0 g/kg for consecutive eight days. Blood and livers were collected at the time of 24 h after dosing. RT-PCR was used to analysis of genes of interest. S. mussotii extract ingestion induced Cyp2b10 and inhibited Cyp3a11 at all doses, while increased the expressions of Cyp2e1 at the dose of 0.1 and 0.3 g/kg and decreased the expression gradually at the dose of 1.0 and 3.0 g/kg. For phase-2 enzyme genes, S. mussotii extract increased sulfotransferase (Sult1a1), heme oxygenase-1 (HO-1) and quinone oxidoreductase 1 (Nqo1), but had no effects on glucuronyltransferases (Ugt1a1 and Ugt1a6) and sulfotransferase (Sult1b1). It is very interesting that S. mussotii extract inhibited mRNA levels in the hepatic organic anion-transporting polypetides (Oatp1a1 and Oatp2b1) and induced multidrug resistance-associated proteins, such as Mrp1 and Mrp4 were especially induced at 3.0 mg/kg. The metabolism of S. mussotii extract includes the induction of cytochrome P450 enzyme genes, hepatic transporters and phase-2 enzyme genes. The S. mussotii extracts influenced all types of metabolic drug processing genes and therefore could play a role in producing herbdrug interactions. (C) 2019 Friends Science Publishers
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/59897]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yang, Hongxia,Xiao, Yuancan,Li, Cen,et al. Effects on Expression of Hepatic Phase-1 and Phase-2 Metabolism and Transporter Genes by Swertia mussotii Extract, A Hepatoprotective Herb from Tibetan Traditional Medicine[J]. INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY,2019,21(5).
APA	Yang, Hongxia,Xiao, Yuancan,Li, Cen,Gao, Tingting,Wei, Lixin,&Du, Yuzhi.(2019).Effects on Expression of Hepatic Phase-1 and Phase-2 Metabolism and Transporter Genes by Swertia mussotii Extract, A Hepatoprotective Herb from Tibetan Traditional Medicine.INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY,21(5).
MLA	Yang, Hongxia,et al."Effects on Expression of Hepatic Phase-1 and Phase-2 Metabolism and Transporter Genes by Swertia mussotii Extract, A Hepatoprotective Herb from Tibetan Traditional Medicine".INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY 21.5(2019).