Aggregation-induced emission enhancement of gold nanoclusters triggered by silicon nanoparticles for ratiometric detection of protamine and trypsin | |
Xue, Fangfang; Qu, Fei; Han, Wenli; Xia, Lian; You, Jinmao | |
刊名 | ANALYTICA CHIMICA ACTA |
2019 | |
卷号 | 1046 |
关键词 | Glutathione-capped gold nanoclusters Silicon nanoparticles Aggregation-induced emission Ratiometric detection Self-assembly |
英文摘要 | Metal nanoclusters protected by glutathione (GSH) have attracted a wide attention due to the unique aggregation-induced emission (AIE) feature. However, the "trigger" effects of ethanol, temperature, pH values, and metal ions may restrict the application of these particles. In this work, the amino modified silicon nanoparticles (SiNPs) and GSH-capped gold nanoclusters (GSH-AuNCs) can self-assemble into well-defined spherical particles due to the electrostatic interaction. As a result, the unique aggregation-induced emission enhancement (AIEE) of GSH-AuNCs arises at 570 nm, and the SiNPs keep their own blue fluorescence at 450 nm, so a novel nanohybrid probe (SiNPs@GSH-AuNCs) with dual-emission property has been constructed. When protamine is added to SiNPs@GSH-AuNCs, the cationic protamine can compete with SiNPs and absorb onto the surface of GSH-AuNCs, which inhibits the self-assembly and leads to the fluorescence quenching of GSH-AuNCs; while trypsin can catalyze the hydrolysis of protamine, the self-assembly starts again, producing the AIEE recovery. In the whole process, the SiNPs act as an internal standard and their emission stays constant. By means of the fluorescence intensity ratios I-570/I-450, the linear range of protamine is from 0.15 to 3.00 mu gmL(-1) with the limit of detection (LOD) of 0.07 mu gmL(-1), and trypsin shows a linear response in the range from 10 to 100 ngmL(-1) with LOD of 4.50 ngmL(-1). Furthermore, this strategy exhibits good sensitivity and selectivity, and has been further validated by applying it for the determination of protamine and trypsin in serum samples. (C) 2018 Elsevier B.V. All rights reserved. |
内容类型 | 期刊论文 |
源URL | [http://210.75.249.4/handle/363003/59877] |
专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Xue, Fangfang,Qu, Fei,Han, Wenli,et al. Aggregation-induced emission enhancement of gold nanoclusters triggered by silicon nanoparticles for ratiometric detection of protamine and trypsin[J]. ANALYTICA CHIMICA ACTA,2019,1046. |
APA | Xue, Fangfang,Qu, Fei,Han, Wenli,Xia, Lian,&You, Jinmao.(2019).Aggregation-induced emission enhancement of gold nanoclusters triggered by silicon nanoparticles for ratiometric detection of protamine and trypsin.ANALYTICA CHIMICA ACTA,1046. |
MLA | Xue, Fangfang,et al."Aggregation-induced emission enhancement of gold nanoclusters triggered by silicon nanoparticles for ratiometric detection of protamine and trypsin".ANALYTICA CHIMICA ACTA 1046(2019). |
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