Comparative transcriptome analysis of two selenium-accumulating genotypes of Aegilops tauschii Coss. in response to selenium

	Comparative transcriptome analysis of two selenium-accumulating genotypes of Aegilops tauschii Coss. in response to selenium
	Wu, Lijun; Liu, Tao; Xu, Yongsheng; Chen, Wenjie; Liu, Baolong; Zhang, Lianquan; Liu, Dengcai; Zhang, Huaigang; Zhang, Bo
刊名	BMC GENETICS
	2019
卷号	20
关键词	Differentially expressed genes Selenium Aegilops tauschii
英文摘要	BackgroundSelenium (Se), an essential micronutrient in both animals and humans, has various biological functions, and its deficiency can lead to various diseases. The most common method for increasing Se uptake is the consumption of Se-rich plants, which transform inorganic Se into organic forms. Wheat is eaten daily by many people. The Se content of Aegilops tauschii (Ae. tauschii), one of the ancestors of hexaploid common wheat, is generally higher than that of wheat. In this study, two genotypes of Ae. tauschii with contrasting Se-accumulating abilities were subjected to different Se treatments followed by high-throughput transcriptome sequencing.ResultsSequencing of 12 transcriptome libraries of Ae. tauschii grown under different Se treatments produced about a total of 47.72GB of clean reads. After filtering out rRNA sequences, approximately 19.3 million high-quality clean reads were mapped to the reference genome (ta IWGSC_MIPSv2.1 genome DA). The total number of reference genome gene is 32,920 and about 26,407 known genes were detected in four groups. Functional annotation of these mapped genes revealed a large number of genes and some pivotal pathways that may participate in Se metabolism. The expressions of several genes potentially involved in Se metabolism were confirmed by quantitative real-time PCR.ConclusionsOur study, the first to examine Se metabolism in Ae. tauschii, has provided a theoretical foundation for future elucidation of the mechanism of Se metabolism in this species.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/59876]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wu, Lijun,Liu, Tao,Xu, Yongsheng,et al. Comparative transcriptome analysis of two selenium-accumulating genotypes of Aegilops tauschii Coss. in response to selenium[J]. BMC GENETICS,2019,20.
APA	Wu, Lijun.,Liu, Tao.,Xu, Yongsheng.,Chen, Wenjie.,Liu, Baolong.,...&Zhang, Bo.(2019).Comparative transcriptome analysis of two selenium-accumulating genotypes of Aegilops tauschii Coss. in response to selenium.BMC GENETICS,20.
MLA	Wu, Lijun,et al."Comparative transcriptome analysis of two selenium-accumulating genotypes of Aegilops tauschii Coss. in response to selenium".BMC GENETICS 20(2019).