Phenotype and temperature affect the affinity for dissolved inorganic carbon in a cyanobacterium Microcystis

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	Phenotype and temperature affect the affinity for dissolved inorganic carbon in a cyanobacterium Microcystis
	Wu, Xinghua 1,2; Wu, Zhongxing 1,3; Song, Lirong 1
刊名	HYDROBIOLOGIA
	2011-10-01
卷号	675 期号:1 页码:175-186
关键词	Phenotype Colonial Microcystis Dissolved inorganic carbon Carbonic anhydrase
ISSN号	0018-8158
通讯作者	Song, LR (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China ; lrsong@ihb.ac.cn
中文摘要	The cyanobacterium Microcystis is the most common bloom-forming species in eutrophicated water bodies. Known eco-physiological advantages of this organism help it to compete effectively with other algae and cyanobacteria; however, little is known about the physiological characteristics competence of colonial Microcystis. In the present study, carbonic anhydrase (CA) activity, the affinity for dissolved inorganic carbon (DIC), and the transcription of CA genes were examined in unicellular and colonial Microcystis strains. In comparison with unicellular strains, colonial Microcystis exhibited dramatically higher inorganic carbon affinity at 25 and 35A degrees C, but no significant differences were observed at 15A degrees C. The relative transcription levels of the CA genes icfA1, icfA2, ecaA, and ecaB in all colonial Microcystis were significantly higher than those in unicellular Microcystis at 25 and 35A degrees C. In addition, CA activities of Microcystis increased with temperature, but no significant difference was observed between the unicellular and colonial Microcystis. These results suggest that temperature and phenotypes probably play important roles in the utilization of DIC and trigger the expression of CA genes of Microcystis. The present results may indicate that the capacity for utilizing inorganic carbon plays a role in the persistence or/and succession of different Microcystis blooms.
英文摘要	The cyanobacterium Microcystis is the most common bloom-forming species in eutrophicated water bodies. Known eco-physiological advantages of this organism help it to compete effectively with other algae and cyanobacteria; however, little is known about the physiological characteristics competence of colonial Microcystis. In the present study, carbonic anhydrase (CA) activity, the affinity for dissolved inorganic carbon (DIC), and the transcription of CA genes were examined in unicellular and colonial Microcystis strains. In comparison with unicellular strains, colonial Microcystis exhibited dramatically higher inorganic carbon affinity at 25 and 35A degrees C, but no significant differences were observed at 15A degrees C. The relative transcription levels of the CA genes icfA1, icfA2, ecaA, and ecaB in all colonial Microcystis were significantly higher than those in unicellular Microcystis at 25 and 35A degrees C. In addition, CA activities of Microcystis increased with temperature, but no significant difference was observed between the unicellular and colonial Microcystis. These results suggest that temperature and phenotypes probably play important roles in the utilization of DIC and trigger the expression of CA genes of Microcystis. The present results may indicate that the capacity for utilizing inorganic carbon plays a role in the persistence or/and succession of different Microcystis blooms.
学科主题	Marine & Freshwater Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Marine & Freshwater Biology
研究领域[WOS]	Marine & Freshwater Biology
关键词[WOS]	CO2 CONCENTRATING MECHANISMS ; DIFFUSIVE BOUNDARY-LAYERS ; SYNECHOCOCCUS PCC7942 ; CO2-CONCENTRATING MECHANISM ; CHLAMYDOMONAS-REINHARDTII ; ANHYDRASE ACTIVITY ; WILD-TYPE ; PHYSIOLOGICAL-RESPONSES ; SYNECHOCYSTIS PCC6803 ; HCO3-TRANSPORT
收录类别	SCI
资助信息	National Key Project for Basic Research[2008CB418006]; NSFC-Yunnan Joint Foundation[U08333604]; Chinese Academy of Sciences[KZCX1-YW-14-1]
语种	英语
WOS记录号	WOS:000293643700017
公开日期	2011-11-09
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/16363]
专题	水生生物研究所_藻类生物学及应用研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Southwest Univ, Key Lab Ecoenvironm Three Gorges Reservoir Reg, Sch Life Sci,Minist Educ, Chongqing Key Lab Plant Ecol & Resources Res Thre, Chongqing 400715, Peoples R China
推荐引用方式 GB/T 7714	Wu, Xinghua,Wu, Zhongxing,Song, Lirong. Phenotype and temperature affect the affinity for dissolved inorganic carbon in a cyanobacterium Microcystis[J]. HYDROBIOLOGIA,2011,675(1):175-186.
APA	Wu, Xinghua,Wu, Zhongxing,&Song, Lirong.(2011).Phenotype and temperature affect the affinity for dissolved inorganic carbon in a cyanobacterium Microcystis.HYDROBIOLOGIA,675(1),175-186.
MLA	Wu, Xinghua,et al."Phenotype and temperature affect the affinity for dissolved inorganic carbon in a cyanobacterium Microcystis".HYDROBIOLOGIA 675.1(2011):175-186.