Toxicogenomic investigation of Tetrahymena thermophila exposed to dichlorodiphenyltrichloroethane (DDT), tributyltin (TBT), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物分子与细胞生物学研究中心 > 期刊论文

	Toxicogenomic investigation of Tetrahymena thermophila exposed to dichlorodiphenyltrichloroethane (DDT), tributyltin (TBT), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)
	Chang Yue 1,3; Feng LiFang 1,2; Miao Wei 1
刊名	SCIENCE CHINA-LIFE SCIENCES
	2011-07-01
卷号	54 期号:7 页码:617-625
关键词	microarray enrichment analysis gene interaction network pollutant
ISSN号	1674-7305
通讯作者	Miao Wei，Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China ; miaowei@ihb.ac.cn
中文摘要	Dichlorodiphenyltrichloroethane (DDT), tributyltin (TBT), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) are persistent in the environment and cause continuous toxic effects in humans and aquatic life. Tetrahymena thermophila has the potential for use as a model for research regarding toxicants. In this study, this organism was used to analyze a genome-wide microarray generated from cells exposed to DDT, TBT and TCDD. To accomplish this, genes differentially expressed when treated with each toxicant were identified, after which their functions were categorized using GO enrichment analysis. The results suggested that the responses of T. thermophila were similar to those of multicellular organisms. Additionally, the context likelihood of relatedness method (CLR) was applied to construct a TCDD-relevant network. The T-shaped network obtained could be functionally divided into two subnetworks. The general functions of both subnetworks were related to the epigenetic mechanism of TCDD. Based on analysis of the networks, a model of the TCDD effect on T. thermophila was inferred. Thus, Tetrahymena has the potential to be a good unicellular eukaryotic model for toxic mechanism research at the genome level.
英文摘要	Dichlorodiphenyltrichloroethane (DDT), tributyltin (TBT), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) are persistent in the environment and cause continuous toxic effects in humans and aquatic life. Tetrahymena thermophila has the potential for use as a model for research regarding toxicants. In this study, this organism was used to analyze a genome-wide microarray generated from cells exposed to DDT, TBT and TCDD. To accomplish this, genes differentially expressed when treated with each toxicant were identified, after which their functions were categorized using GO enrichment analysis. The results suggested that the responses of T. thermophila were similar to those of multicellular organisms. Additionally, the context likelihood of relatedness method (CLR) was applied to construct a TCDD-relevant network. The T-shaped network obtained could be functionally divided into two subnetworks. The general functions of both subnetworks were related to the epigenetic mechanism of TCDD. Based on analysis of the networks, a model of the TCDD effect on T. thermophila was inferred. Thus, Tetrahymena has the potential to be a good unicellular eukaryotic model for toxic mechanism research at the genome level.
学科主题	Life Sciences & Biomedicine - Other Topics
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biology
研究领域[WOS]	Life Sciences & Biomedicine - Other Topics
关键词[WOS]	ARYL-HYDROCARBON RECEPTOR ; SUPPRESSION SUBTRACTIVE HYBRIDIZATION ; GENE-EXPRESSION ; P-GLYCOPROTEIN ; OXIDATIVE STRESS ; RAT-LIVER ; ACTIVATION ; APOPTOSIS ; SEQUENCE ; DEGRADATION
收录类别	SCI
资助信息	National Natural Science Foundation of China[30870356, 31071993]; Chinese Academy of Sciences[KSCX2-YW-G-072]
语种	英语
WOS记录号	WOS:000292700100004
公开日期	2011-11-09
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/16359]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China 2.Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Hangzhou 310035, Zhejiang, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Chang Yue,Feng LiFang,Miao Wei. Toxicogenomic investigation of Tetrahymena thermophila exposed to dichlorodiphenyltrichloroethane (DDT), tributyltin (TBT), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)[J]. SCIENCE CHINA-LIFE SCIENCES,2011,54(7):617-625.
APA	Chang Yue,Feng LiFang,&Miao Wei.(2011).Toxicogenomic investigation of Tetrahymena thermophila exposed to dichlorodiphenyltrichloroethane (DDT), tributyltin (TBT), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).SCIENCE CHINA-LIFE SCIENCES,54(7),617-625.
MLA	Chang Yue,et al."Toxicogenomic investigation of Tetrahymena thermophila exposed to dichlorodiphenyltrichloroethane (DDT), tributyltin (TBT), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)".SCIENCE CHINA-LIFE SCIENCES 54.7(2011):617-625.