Mask-free generation of multicellular 3D heterospheroids array for high-throughput combinatorial anti-cancer drug screening | |
Yang WG(杨文广)4; Lai YB(来佑彬)3; Yu HB(于海波)1,2; Wang YC(王越超)1,2; Yuan, Z.4; Cai, Shuxiang4 | |
刊名 | Materials and Design |
2019 | |
卷号 | 183页码:1-8 |
关键词 | 3D heterospheroids generation microwells fabrication drug screening |
ISSN号 | 0264-1275 |
产权排序 | 3 |
英文摘要 | Since cancer incidence and mortality are rapidly growing worldwide, it is imperative to develop the effective tumor models in vitro for anti-cancer drug screening. Cellular spheroid as an excellent simple 3D model can bridge the gap between cell culture and live tissue. Although numerous approaches for generating cellular spheroids emerges, the long cultivation time and the heterospheroids formation with multiple cell types are still challenging. In the present study, a mask-free method of multicellular heterospheroids arrays formation using digital micromirror device (DMD)-based hydrogel fabricating system and microfluidic system is proposed. Microwell arrays were designed and manufactured online based on shadowed light which shows high flexible. Besides, heterospheroids with multiple cells controlled in different ratios can be generated and cultured for a long time in microfluidic system. Furthermore, the drug screening shows that heterospheroids have high rate of drug resistance compared with homospheroids and combinatorial drugs works better than the single drugs. These studies provide a 3D heterospheroids generating process for high-throughput combinatorial anti-cancer drug screening with potential cancer therapeutic application. |
语种 | 英语 |
WOS记录号 | WOS:000490732800032 |
资助机构 | National Natural Science Foundation of China (Project No. 61803323 and 51605311) ; Shandong Province Natural Science Foundation (Project No. ZR2019BF049) |
内容类型 | 期刊论文 |
源URL | [http://ir.sia.cn/handle/173321/25626] |
专题 | 沈阳自动化研究所_机器人学研究室 |
通讯作者 | Yang WG(杨文广) |
作者单位 | 1.Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110016, China 2.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China 3.College of Engineering, Shenyang Agricultural University, Shenyang 110016, China 4.School of Electromechanical and Automotive Engineering, Yantai University, Yantai 264005, China |
推荐引用方式 GB/T 7714 | Yang WG,Lai YB,Yu HB,et al. Mask-free generation of multicellular 3D heterospheroids array for high-throughput combinatorial anti-cancer drug screening[J]. Materials and Design,2019,183:1-8. |
APA | Yang WG,Lai YB,Yu HB,Wang YC,Yuan, Z.,&Cai, Shuxiang.(2019).Mask-free generation of multicellular 3D heterospheroids array for high-throughput combinatorial anti-cancer drug screening.Materials and Design,183,1-8. |
MLA | Yang WG,et al."Mask-free generation of multicellular 3D heterospheroids array for high-throughput combinatorial anti-cancer drug screening".Materials and Design 183(2019):1-8. |
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