Radiation-induced cellular senescence results from a slippage of long-term G(2) arrested cells into G(1) phase

doi:10.4161/cc.24528

	Radiation-induced cellular senescence results from a slippage of long-term G(2) arrested cells into G(1) phase
	Ye, Caiyong 1,2; Wang, Jufang2 ; Zhang, Xurui 1,2; Zhou, Guangming2 ; Wan, Jianghua 2; Chang, Lei 1,2; Hu, Wentao 1,2; Bing, Zhitong 2; Zhang, Sheng 1,2; Li, Junhong 1,2
刊名	CELL CYCLE
	2013-05-01
卷号	12 页码:1424-1432
关键词	Ionizing Radiation g(2) Slippage Senescence Cell Cycle Checkpoint Dna Damage Response Mitotic Slippage
ISSN号	1538-4101
DOI	10.4161/cc.24528
文献子类	Article
英文摘要	Diploid cells undergoing senescence and mitotic slippage have been reported in the literature. However, the mechanisms triggering senescence in long-term G(2)-arrested cells are currently unclear. Previously, we reported that the cell cycle of the human uveal melanoma cell line, 92-1, is suspended for up to 6 d upon exposure to 10 Gy ionizing radiation (IR), followed by senescence. In the current study, we initially distinguished senescence in long-term blocked 92-1 cells from mitotic slippage by confirming the blockage of cells in the G(2) phase. We subsequently showed that the genes essential for G(2)-M transition are prematurely downregulated at both the transcriptional and translational levels. Furthermore, levels of the G(1)-specific markers, Cyclin D1 and Caveolin-1, were distinctly increased, while S/G(2)-specific markers, Cyclin B1 and Aurora A, were significantly downregulated. These findings collectively imply that long-term G(2)-arrested cells undergo senescence via G(2) slippage. To our knowledge, this is the first study to report that the cellular process of G(2) slippage is the mechanism responsible for senescence of cells under long-term G(2) arrest.
WOS关键词	HUMAN DIPLOID CELLS ; CYCLIN D1 EXPRESSION ; DNA-DAMAGE-RESPONSE ; MAMMALIAN-CELLS ; CANCER ; POLYPLOIDY ; CHECKPOINT ; ENDOREPLICATION ; ACTIVATION ; G2
语种	英语
出版者	LANDES BIOSCIENCE
WOS记录号	WOS:000322758600017
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/48297]
专题	近代物理研究所_生物物理研究室
通讯作者	Wang, Jufang
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Modem Phys, Dept Space Radiobiol, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Peoples R China
推荐引用方式 GB/T 7714	Ye, Caiyong,Wang, Jufang,Zhang, Xurui,et al. Radiation-induced cellular senescence results from a slippage of long-term G(2) arrested cells into G(1) phase[J]. CELL CYCLE,2013,12:1424-1432.
APA	Ye, Caiyong.,Wang, Jufang.,Zhang, Xurui.,Zhou, Guangming.,Wan, Jianghua.,...&He, Jinpeng.(2013).Radiation-induced cellular senescence results from a slippage of long-term G(2) arrested cells into G(1) phase.CELL CYCLE,12,1424-1432.
MLA	Ye, Caiyong,et al."Radiation-induced cellular senescence results from a slippage of long-term G(2) arrested cells into G(1) phase".CELL CYCLE 12(2013):1424-1432.