Changes in the physiological properties and kinetics of citric acid accumulation via carbon ion irradiation mutagenesis of Aspergillus niger

doi:10.1631/jzus.B1500120

	Changes in the physiological properties and kinetics of citric acid accumulation via carbon ion irradiation mutagenesis of Aspergillus niger
	Li, Wen-jian1,2 ; Wu, Qing-feng2 ; Liu, Jing1,2 ; Hu, Wei 1,2; Song, Yuan 1,2; Chen, Ji-hong1,2 ; Wang, Shu-yang1,2
刊名	JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
	2016-04-01
卷号	17 页码:262-270
关键词	Carbon ion irradiation Physiological properties Mutation Citric acid accumulation
ISSN号	1673-1581
DOI	10.1631/jzus.B1500120
英文摘要	The objective of this work was to produce citric acid from corn starch using a newly isolated mutant of Aspergillus niger, and to analyze the relationship between changes in the physiological properties of A. niger induced by carbon ion irradiation and citric acid accumulation. Our results showed that the physiological characteristics of conidia in A. niger were closely related to citric acid accumulation and that lower growth rate and viability of conidia may be beneficial to citric acid accumulation. Using corn starch as a raw material, a high-yielding citric acid mutant, named HW2, was obtained. In a 10-L bioreactor, HW2 can accumulate 118.9 g/L citric acid with a residual total sugar concentration of only 14.4 g/L. This represented an 18% increase in citric acid accumulation and a 12.5% decrease in sugar utilization compared with the original strain.
资助项目	Agriculture Science Technology Achievement Transformation Fund, China[2013GB24910680]
WOS关键词	TEMPERATURE PLASMA ARTP ; SOLID-STATE FERMENTATION ; STRAIN IMPROVEMENT ; ARABIDOPSIS-THALIANA ; MUTANT STRAIN ; MUTATION ; MORPHOLOGY ; STARCH ; GROWTH ; PRODUCTIVITY
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Research & Experimental Medicine
语种	英语
出版者	ZHEJIANG UNIV
WOS记录号	WOS:000374298000002
资助机构	Agriculture Science Technology Achievement Transformation Fund, China
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/42389]
专题	近代物理研究所_生物物理研究室
通讯作者	Li, Wen-jian; Chen, Ji-hong
作者单位	1.Key Lab Microbial Resources Exploit & Applicat, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Li, Wen-jian,Wu, Qing-feng,Liu, Jing,et al. Changes in the physiological properties and kinetics of citric acid accumulation via carbon ion irradiation mutagenesis of Aspergillus niger[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2016,17:262-270.
APA	Li, Wen-jian.,Wu, Qing-feng.,Liu, Jing.,Hu, Wei.,Song, Yuan.,...&Wang, Shu-yang.(2016).Changes in the physiological properties and kinetics of citric acid accumulation via carbon ion irradiation mutagenesis of Aspergillus niger.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,17,262-270.
MLA	Li, Wen-jian,et al."Changes in the physiological properties and kinetics of citric acid accumulation via carbon ion irradiation mutagenesis of Aspergillus niger".JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B 17(2016):262-270.