Spectroscopic assessment of argon gas discharge induced radiolysis of aqueous adenine and thymine

doi:10.1016/j.radphyschem.2011.06.017

	Spectroscopic assessment of argon gas discharge induced radiolysis of aqueous adenine and thymine
	Dang, Bingrong1 ; Wang, Xiangqin 2; Yu, Zengliang 2; Su, Xi 2; Huang, Qing 2
刊名	RADIATION PHYSICS AND CHEMISTRY
	2011-12-01
卷号	80 页码:1343-1351
关键词	Plasma discharge Radiolysis Spectroscopy Adenine Thymine Liquid chromatography/mass spectrometry
ISSN号	0969-806X
DOI	10.1016/j.radphyschem.2011.06.017
英文摘要	Ionizing radiation influences life profoundly for it can modify genetic materials. It is a long-standing task to investigate the interaction between energetic particles and DNA together with its components such as nucleotides, nucleosides and bases so as to predict and assess the potential biological effects. In this study, argon gas discharge was employed to produce energetic ions and electrons. The gas discharge caused the radiolysis of aqueous bases and the involved reactions were analyzed by means of spectroscopic tools including UV-vis absorption, fluorescence and Fourier transformation infrared (FTIR) spectroscopy, also assisted by liquid chromatography/mass spectrometry (LC/MS). It was found that the discharge resulted in the adenine-derived lesions such as 4,6-diamino-5-formamidopyrimidine, 8-OH-Ade and 2-OH-Ade in the radiolysis of aqueous adenine, as well as the thymine-derived lesions such as thymine glycol, 5-hydroxy-6-hydrothymine and/or 6-hydroxy-5-hydrothymine, 5-hydroxymethyluracil and 5-formyluracil in the radiolysis of aqueous thymine. The study of radiosensitivity showed that adenine was more resistant to the discharge. The mechanisms of the involved reactions were studied in detail, confirming that the hydroxyl radical played a dominant role. (C) 2011 Elsevier Ltd. All rights reserved.
资助项目	Chinese Academy of Sciences[KJCX2-YW-N34-1] ; Natural Science Foundation of China[10975152]
WOS关键词	TANDEM MASS-SPECTROMETRY ; RADIATION-INDUCED DAMAGE ; OXIDATIVE DNA-DAMAGE ; INDUCED BASE DAMAGE ; IONIZING-RADIATION ; TRANSFORMATION REACTIONS ; GAMMA-RADIOLYSIS ; STRAND BREAKAGE ; NUCLEIC-ACIDS ; CELLULAR-DNA
WOS研究方向	Chemistry ; Nuclear Science & Technology ; Physics
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000295665100009
资助机构	Chinese Academy of Sciences ; Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/33320]
专题	近代物理研究所_生物物理研究室
通讯作者	Huang, Qing
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab Ion Beam Bioengn, Hefei 230031, Peoples R China
推荐引用方式 GB/T 7714	Dang, Bingrong,Wang, Xiangqin,Yu, Zengliang,et al. Spectroscopic assessment of argon gas discharge induced radiolysis of aqueous adenine and thymine[J]. RADIATION PHYSICS AND CHEMISTRY,2011,80:1343-1351.
APA	Dang, Bingrong,Wang, Xiangqin,Yu, Zengliang,Su, Xi,&Huang, Qing.(2011).Spectroscopic assessment of argon gas discharge induced radiolysis of aqueous adenine and thymine.RADIATION PHYSICS AND CHEMISTRY,80,1343-1351.
MLA	Dang, Bingrong,et al."Spectroscopic assessment of argon gas discharge induced radiolysis of aqueous adenine and thymine".RADIATION PHYSICS AND CHEMISTRY 80(2011):1343-1351.