Influence of the environment and phototoxicity of the live cell imaging system at IMP microbeam facility

doi:10.1016/j.nimb.2017.01.076

	Influence of the environment and phototoxicity of the live cell imaging system at IMP microbeam facility
	Wu, Ruqun 1,3; Guo, Jinlong1,2 ; Du, Guanghua1 ; Liu, Wenjing 1; Zhao, Jing 1,3; Wei, Junzhe 1,3; Chen, Hao 4; Li, Yaning 1,3; Li, Xiaoyue 1,3
刊名	NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS
	2017-08-01
卷号	404 页码:125-130
关键词	Live cell imaging DNA damage and repair Phototoxicity Environment control Microbeam
ISSN号	0168-583X
DOI	10.1016/j.nimb.2017.01.076
英文摘要	To investigate the spatiotemporal dynamics of DNA damage and repair after the ion irradiation, an online live cell imaging system has been established based on the microbeam facility at Institute of Modern Physics (IMP). The system could provide a sterile and physiological environment by making use of heating plate and live cell imaging solution. The phototoxicity was investigated through the evaluation of DNA repair protein XRCC1 foci formed in HT1080-RFP cells during the imaging exposure. The intensity of the foci induced by phototoxicity was much lower compared with that of the foci induced by heavy ion hits. The results showed that although spontaneous foci were formed due to RFP exposure during live cell imaging, they had little impact on the analysis of the recruitment kinetics of XRCC1 in the foci induced by the ion irradiation. (C) 2017 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China (NSFC)[31200630] ; National Natural Science Foundation of China (NSFC)[U1632271] ; Ministry of Science and Technology[2012YQ03014204]
WOS关键词	STRAND BREAK REPAIR ; DNA ; DYNAMICS ; PROTEIN
WOS研究方向	Instruments & Instrumentation ; Nuclear Science & Technology ; Physics
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000404709900024
资助机构	National Natural Science Foundation of China (NSFC) ; Ministry of Science and Technology
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/45160]
专题	近代物理研究所_实验物理中心
通讯作者	Du, Guanghua
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China 2.Northwest Normal Univ, Lanzhou, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Univ Lanzhou, Lanzhou, Peoples R China
推荐引用方式 GB/T 7714	Wu, Ruqun,Guo, Jinlong,Du, Guanghua,et al. Influence of the environment and phototoxicity of the live cell imaging system at IMP microbeam facility[J]. NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS,2017,404:125-130.
APA	Wu, Ruqun.,Guo, Jinlong.,Du, Guanghua.,Liu, Wenjing.,Zhao, Jing.,...&Li, Xiaoyue.(2017).Influence of the environment and phototoxicity of the live cell imaging system at IMP microbeam facility.NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS,404,125-130.
MLA	Wu, Ruqun,et al."Influence of the environment and phototoxicity of the live cell imaging system at IMP microbeam facility".NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS 404(2017):125-130.