Radio-resistance induced by nitric oxide to heavy ion irradiation in A172 human glioma cells

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	Radio-resistance induced by nitric oxide to heavy ion irradiation in A172 human glioma cells
	Zhou Qingming 2,3; Zhang Hong2 ; Zhang Xingxia 1
刊名	NUCLEAR SCIENCE AND TECHNIQUES
	2007-10-20
卷号	18 期号:5 页码:282-286
关键词	nitric oxide C-12(6+) heavy ion A172 human glioma cells radio-resistance
ISSN号	1001-8042
英文摘要	To investigate effects of nitric oxide on cellular radio-sensitivity, three human glioma cell lines, i.e. A172, A172 transfected green fluorescence protein (EGFP) gene (EA172) and A172 transfected inducible nitric oxide synthesis (iNOS) gene (iA72), were irradiated by C-12(6+) ions to 0, 1 or My. Productions of nitric oxide and glutathione (GSH) in A172, EA172 and iA172 were determined by chemical methods, cell cycle was analyzed by flow cytometry at the 24th hour after irradiation, and survival fraction of the cells was measured by colorimetric MTT assay at the 5th day after irradiation. The results showed that the concentrations of nitric oxide and GSH in iA172 were significantly higher than in A172 and EA172; the G(2)/M stage arrest induced by the C-12(6+) ion irradiation was observed in A172 and EA172 but not in iA172 at the 24th hour after exposure; and the survival fraction of iA172 was higher than that of EA172 and iA172. Data suggest that the radio-sensitivity of the A172 was reduced after the iNOS gene transfection. The increase of GSH production and the change of cellular signals such as the cell cycle control induced by nitric oxide may be involved in this radio-resistance.
WOS关键词	RADIORESISTANCE ; INDUCTION ; P53
WOS研究方向	Nuclear Science & Technology ; Physics
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000255053100006
公开日期	2010-10-29
内容类型	期刊论文
源URL	[http://ir.imp.cas.cn/handle/113462/6335]
专题	近代物理研究所_近代物理研究所知识存储（2010之前）
通讯作者	Zhang Hong
作者单位	1.Lanzhou Univ, Hosp 1st, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhou Qingming,Zhang Hong,Zhang Xingxia. Radio-resistance induced by nitric oxide to heavy ion irradiation in A172 human glioma cells[J]. NUCLEAR SCIENCE AND TECHNIQUES,2007,18(5):282-286.
APA	Zhou Qingming,Zhang Hong,&Zhang Xingxia.(2007).Radio-resistance induced by nitric oxide to heavy ion irradiation in A172 human glioma cells.NUCLEAR SCIENCE AND TECHNIQUES,18(5),282-286.
MLA	Zhou Qingming,et al."Radio-resistance induced by nitric oxide to heavy ion irradiation in A172 human glioma cells".NUCLEAR SCIENCE AND TECHNIQUES 18.5(2007):282-286.