Ginsenoside R-e increases fertile and asthenozoospermic infertile human sperm motility by induction of nitric oxide synthase

CORC > 近代物理研究所 > 中国科学院近代物理研究所 > 近代物理研究所知识存储（2010之前）

	Ginsenoside R-e increases fertile and asthenozoospermic infertile human sperm motility by induction of nitric oxide synthase
	Zhang, H; Zhou, QM; Li, XD; Xie, Y; Duan, X; Min, FL; Liu, B; Yuan, ZG
刊名	ARCHIVES OF PHARMACAL RESEARCH
	2006-02-01
卷号	29 期号:2 页码:145-151
关键词	ginsenoside R-e sperm motility nitric oxide synthase nitric oxide reactive oxygen species
ISSN号	0253-6269
英文摘要	We investigated the effects of Ginsenoside R-e on human sperm motility in fertile and asthenozoospermic infertile individuals in vitro and the mechanism by which the Ginsenosides play their roles. The semen samples were obtained from 10 fertile volunteers and 10 asthenozoospermic infertile patients. Spermatozoa were separated by Percoll and incubated with 0, 1, 10 or 100 mu M of Ginsenoside R-e. Total sperm motility and progressive motility were measured by computer-aided sperm analyzer (CASA). Nitric oxide synthase (NOS) activity was determined by the H-3-arginine to H-3-citrulline conversion assay, and the NOS protein was examined by the Western blot analysis. The production of sperm nitric oxide (NO) was detected using the Griess reaction. The results showed that Ginsenoside R-e significantly enhanced both fertile and infertile sperm motility, NOS activity and NO production in a concentration-dependent manner. Sodium nitroprusside (SNP, 100 nM), a NO donor, mimicked the effects of Ginsenoside R-e. And pretreatment with a NOS inhibitor N-omega-Nitro-L-arginine methyl ester (L-NAME, 100 mu M) or a NO scavenger N-Acetyl-L-cysteine (LNAC, 1 mM) completely blocked the effects of Ginsenoside R-e. Data suggested that Ginsenoside R-e is beneficial to sperm motility, and that induction of NOS to increase NO production may be involved in this benefit.
WOS关键词	HUMAN-SPERMATOZOA ; LIPID-PEROXIDATION ; IN-VITRO ; TYROSINE PHOSPHORYLATION ; VENTRICULAR MYOCYTES ; SUPEROXIDE ANION ; PANAX-GINSENG ; RAT ; CAPACITATION ; PHARMACOLOGY
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	PHARMACEUTICAL SOCIETY KOREA
WOS记录号	WOS:000235691200006
公开日期	2010-10-29
内容类型	期刊论文
源URL	[http://ir.imp.cas.cn/handle/113462/6281]
专题	近代物理研究所_近代物理研究所知识存储（2010之前）
通讯作者	Zhang, H
作者单位	Chinese Acad Sci, Inst Modern Phys, Dept Med Phys, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Zhang, H,Zhou, QM,Li, XD,et al. Ginsenoside R-e increases fertile and asthenozoospermic infertile human sperm motility by induction of nitric oxide synthase[J]. ARCHIVES OF PHARMACAL RESEARCH,2006,29(2):145-151.
APA	Zhang, H.,Zhou, QM.,Li, XD.,Xie, Y.,Duan, X.,...&Yuan, ZG.(2006).Ginsenoside R-e increases fertile and asthenozoospermic infertile human sperm motility by induction of nitric oxide synthase.ARCHIVES OF PHARMACAL RESEARCH,29(2),145-151.
MLA	Zhang, H,et al."Ginsenoside R-e increases fertile and asthenozoospermic infertile human sperm motility by induction of nitric oxide synthase".ARCHIVES OF PHARMACAL RESEARCH 29.2(2006):145-151.