Biodosimetry estimate for high-LET irradiation

doi:10.1007/s00411-007-0110-7

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	Biodosimetry estimate for high-LET irradiation
	Wang, Z. Z.; Li, W. J.; Zhi, D. J.; Jing, X. G.; Wei, W.; Gao, Q. X.; Liu, B.
刊名	RADIATION AND ENVIRONMENTAL BIOPHYSICS
	2007-08-01
卷号	46 期号:3 页码:229-235
ISSN号	0301-634X
DOI	10.1007/s00411-007-0110-7
英文摘要	The purpose of this paper is to prepare for an easy and reliable biodosimeter protocol for radiation accidents involving high-linear energy transfer (LET) exposure. Human peripheral blood lymphocytes were irradiated using carbon ions (LET: 34.6 keV mu m(-1)), and the chromosome aberrations induced were analyzed using both a conventional colcemid block method and a calyculin A induced premature chromosome condensation (PCC) method. At a lower dose range (0-4 Gy), the measured dicentric (dics) and centric ring chromosomes (cRings) provided reasonable dose information. At higher doses (8 Gy), however, the frequency of dics and cRings was not suitable for dose estimation. Instead, we found that the number of Giemsa-stained drug-induced G2 prematurely condensed chromosomes (G2-PCC) can be used for dose estimation, since the total chromosome number (including fragments) was linearly correlated with radiation dose (r = 0.99). The ratio of the longest and the shortest chromosome length of the drug-induced G2-PCCs increased with radiation dose in a linear-quadratic manner (r = 0.96), which indicates that this ratio can also be used to estimate radiation doses. Obviously, it is easier to establish the dose response curve using the PCC technique than using the conventional metaphase chromosome method. It is assumed that combining the ratio of the longest and the shortest chromosome length with analysis of the total chromosome number might be a valuable tool for rapid and precise dose estimation for victims of radiation accidents.
WOS关键词	PREMATURE CHROMOSOME CONDENSATION ; DOSE RADIATION-EXPOSURE ; TIME-COURSE ; V79 CELLS ; ABERRATIONS ; INTERPHASE ; DAMAGE ; METAPHASE ; NUMBER ; SPACE
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Biophysics ; Environmental Sciences & Ecology ; Radiology, Nuclear Medicine & Medical Imaging
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000248306200004
公开日期	2010-10-29
内容类型	期刊论文
源URL	[http://ir.imp.cas.cn/handle/113462/5909]
专题	近代物理研究所_近代物理研究所知识存储（2010之前）
通讯作者	Wang, Z. Z.
作者单位	1.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Radiobiol Lab, Lanzhou 730000, Gansu, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Z. Z.,Li, W. J.,Zhi, D. J.,et al. Biodosimetry estimate for high-LET irradiation[J]. RADIATION AND ENVIRONMENTAL BIOPHYSICS,2007,46(3):229-235.
APA	Wang, Z. Z..,Li, W. J..,Zhi, D. J..,Jing, X. G..,Wei, W..,...&Liu, B..(2007).Biodosimetry estimate for high-LET irradiation.RADIATION AND ENVIRONMENTAL BIOPHYSICS,46(3),229-235.
MLA	Wang, Z. Z.,et al."Biodosimetry estimate for high-LET irradiation".RADIATION AND ENVIRONMENTAL BIOPHYSICS 46.3(2007):229-235.