NADPH oxidase-mediated generation of reactive oxygen species: A new mechanism for X-ray-induced HeLa cell death

doi:10.1016/j.bbrc.2008.10.067

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	NADPH oxidase-mediated generation of reactive oxygen species: A new mechanism for X-ray-induced HeLa cell death
	Liu, Qing 2,3; He, Xiaoqing 4; Liu, Yongsheng 5; Du, Bingbing 6; Wang, Xiaoyan 6; Zhang, Weisheng 7; Jia, Pengfei 1; Dong, Jingmei 8; Ma, Jianxiu 9; Wang, Xiaohu 10
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2008-12-19
卷号	377 期号:3 页码:775-779
关键词	NADPH oxidase New mechanism X-ray irradiation HeLa cell death
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2008.10.067
英文摘要	Oxidative damage is an important mechanism in X-ray-induced cell death. Radiolysis of water molecules is a source of reactive oxygen species (ROS) that contribute to X-ray-induced cell death. In this study, we showed by ROS detection and a cell survival assay that NADPH oxidase has a very important role in X-ray-induced cell death. Under X-ray irradiation, the upregulation of the expression of NADPH oxidase membrane Subunit gp91(phox) was dose-dependent. Meanwhile, the cytoplasmic subunit p47(phox) was translocated to the cell membrane and localized with p22(phox) and gp91(phox) to form reactive NADPH oxidase. Our data Suggest, for the first time, that NADPH oxidase-mediated generation of ROS is an important contributor to X-ray-induced cell death. This suggests a new target for combined gene transfer and radiotherapy. (C) 2008 Elsevier Inc. All rights reserved.
资助项目	National Natural Science Foundation of China[10675151] ; National Natural Science Foundation of China[30870586] ; Postdoctoral Foundation of China[20070410232] ; Lanzhou Prajna Union Biology Technique Co., Ltd
WOS关键词	OXIDATIVE STRESS ; MAMMALIAN-CELLS ; IN-VITRO ; RADIOSENSITIZATION ; CARCINOMA ; ACTIVATION ; EXPRESSION
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000261458900008
资助机构	National Natural Science Foundation of China ; Postdoctoral Foundation of China ; Lanzhou Prajna Union Biology Technique Co., Ltd
公开日期	2010-10-29
内容类型	期刊论文
源URL	[http://ir.imp.cas.cn/handle/113462/5521]
专题	近代物理研究所_近代物理研究所知识存储（2010之前）
通讯作者	Zhang, Hong
作者单位	1.Lanzhou Univ, Sch Life Sci, Inst Cell Biol, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, Dept Radiobiol, Lanzhou 730000, Peoples R China 3.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou 730000, Peoples R China 4.Lanzhou Univ, Affiliated Hosp 1, Lanzhou 730000, Peoples R China 5.Chinese Acad Agr, Lanzhou Vet Res Inst, Minist Agr, Key Lab Anim Virol, Lanzhou 730046, Peoples R China 6.Lanzhou Univ, Coll Med, Lanzhou 730000, Peoples R China 7.Gansu Prov Peoples Hosp, Lanzhou 730000, Peoples R China 8.Lanzhou City Univ, Inst Sports Med, Lanzhou 730070, Peoples R China 9.NW Univ Nationalities, Coll Med, Lanzhou 730030, Peoples R China 10.Gansu Prov Canc Hosp, Dept Radiol, Lanzhou 730050, Peoples R China
推荐引用方式 GB/T 7714	Liu, Qing,He, Xiaoqing,Liu, Yongsheng,et al. NADPH oxidase-mediated generation of reactive oxygen species: A new mechanism for X-ray-induced HeLa cell death[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2008,377(3):775-779.
APA	Liu, Qing.,He, Xiaoqing.,Liu, Yongsheng.,Du, Bingbing.,Wang, Xiaoyan.,...&Zhang, Hong.(2008).NADPH oxidase-mediated generation of reactive oxygen species: A new mechanism for X-ray-induced HeLa cell death.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,377(3),775-779.
MLA	Liu, Qing,et al."NADPH oxidase-mediated generation of reactive oxygen species: A new mechanism for X-ray-induced HeLa cell death".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 377.3(2008):775-779.