Dynamic recognition and repair of DNA complex damage

doi:10.1002/jcp.27971

	Dynamic recognition and repair of DNA complex damage
	Yan, Junfang 1,2,3,4; Xie, Yi2,3,4 ; Zhang, Qianjing 1,2,3,4; Gan, Lu1,2,3,4 ; Wang, Fang 1,2,3,4; Li, Hongyan 2,3,4; Si, Jing2,3,4 ; Zhang, Hong2,3,4
刊名	JOURNAL OF CELLULAR PHYSIOLOGY
	2019-08-01
卷号	234 期号:8 页码:13014-13020
关键词	bleomycin carbon ion irradiation DNA damage
ISSN号	0021-9541
DOI	10.1002/jcp.27971
通讯作者	Zhang, Hong(zhangh@impcas.ac.cn)
英文摘要	Irradiation (IR) can be used to treat cancer by inducing complex and irreparable DNA damage in the cancer cells, which may lead to their apoptotic death. However, little is known about the molecular mechanism of this DNA damage. Here, the non-small-cell lung cancer cell line A549 was treated with either X-ray or carbon ion combined with bleomycin (BLM). The cell survival rate, frequency of double-strand breaks (DSBs), dynamic changes in H2AX, and p53 binding protein 1 (53BP1), and protein expression of Ku70, Rad51, and XRCC1 were determined by the clone formation assay, agarose gel electrophoresis, immunofluorescence, and western blot analysis. The results showed that the most obvious complex DSBs occurred in the carbon IR+BLM group. The number of H2AX and 53BP1 foci in the 0.5hr X-ray IR+BLM group was the highest (p<0.001) among all the groups. H2AX foci were detected in the nucleus at 0.5, 1, 2, and 4hr, but were distributed throughout the cell at 6hr after IR in the carbon ion IR+BLM group. The expression of Ku70 increased and XRCC1 decreased at 2 and 6hr after IR. Our data indicate that a DNA damage frequency of 13.4/Mbp is caused by clustered DNA damage and further show a correlation between H2AX, 53BP1, and XRCC1 levels and the extent of DNA damage. The results of this study provide insights into DNA damage recognition and a rationale for the clinical use of radiotherapy.
资助项目	Ministry of science and technology national key R D project[2016YFC0904602] ; Key Program of National Natural Science Foundation of China[U1432248] ; National Natural Science Foundation of China[11605260] ; CAS Light of West China Program
WOS关键词	CARBON-ION ; IN-VITRO ; RADIATION ; BLEOMYCIN ; BREAKAGE ; INDUCE ; ENERGY ; CELLS ; HETEROCHROMATIN ; RADIOTHERAPY
WOS研究方向	Cell Biology ; Physiology
语种	英语
出版者	WILEY
WOS记录号	WOS:000467240800079
资助机构	Ministry of science and technology national key R D project ; Key Program of National Natural Science Foundation of China ; National Natural Science Foundation of China ; CAS Light of West China Program
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/133687]
专题	中国科学院近代物理研究所
通讯作者	Zhang, Hong
作者单位	1.Univ Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China 3.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Gansu, Peoples R China 4.Key Lab Heavy Ion Radiat Med Gansu Prov, Lanzhou, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Yan, Junfang,Xie, Yi,Zhang, Qianjing,et al. Dynamic recognition and repair of DNA complex damage[J]. JOURNAL OF CELLULAR PHYSIOLOGY,2019,234(8):13014-13020.
APA	Yan, Junfang.,Xie, Yi.,Zhang, Qianjing.,Gan, Lu.,Wang, Fang.,...&Zhang, Hong.(2019).Dynamic recognition and repair of DNA complex damage.JOURNAL OF CELLULAR PHYSIOLOGY,234(8),13014-13020.
MLA	Yan, Junfang,et al."Dynamic recognition and repair of DNA complex damage".JOURNAL OF CELLULAR PHYSIOLOGY 234.8(2019):13014-13020.