A genome-wide view of mutations in respiration-deficient mutants of Saccharomyces cerevisiae selected following carbon ion beam irradiation

doi:10.1007/s00253-019-09626-0

	A genome-wide view of mutations in respiration-deficient mutants of Saccharomyces cerevisiae selected following carbon ion beam irradiation
	Guo, Xiaopeng 1,2; Zhang, Miaomiao1,2,3 ; Gao, Yue 1,2; Cao, Guozhen 1,4; Yang, Yang 5; Lu, Dong1,3 ; Li, Wenjian1,3
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2019-02-01
卷号	103 页码:1851-1864
关键词	Saccharomyces cerevisiae Carbon ion beam irradiation Respiration-deficient mutants Genome-wide resequencing Molecular mutation spectrum Mitochondrial genome
ISSN号	0175-7598
DOI	10.1007/s00253-019-09626-0
英文摘要	Mitochondrial dysfunction in Saccharomyces cerevisiae was selected as a marker of ion penetration following carbon ion beam (CIB) irradiation. Respiration-deficient mutants were screened. Following confirmation of negligible spontaneous mutation, eight genetically stable S. cerevisiae respiration-deficient mutant strains and a control strain were resequenced with 200-fold read depth. Strategies were established to identify and validate the particular mutations induced by CIB irradiation. In the nuclear genome, CIB irradiation mainly caused base substitutions and some small (<100bp) insertions/deletions (indels), which were widely distributed across the chromosomes. Although mitochondrial dysfunction was selected as a screening marker, variants in the nuclear genome were detected at a high frequency (10(-7)) relative to spontaneous mutations (10(-9)). The transition to transversion ratio for base substitutions was 0.746, which was less than that of spontaneous mutations. In the mitochondrial genome, there were very large deletions including substantial gene areas, resulting in extremely low read coverage. Meanwhile, every mutant possessed a distinctive mutation pattern, for both the nuclear and the mitochondrial genome. Nuclear genomes contained scanty mitochondrial respiration-related genes that were potentially affected by verified mutations, suggesting that variants in the mitochondrial genome may be the main drivers of respiratory deficiencies. Our study confirmed the previous finding that heavy ion beam (HIB) irradiation mainly induces substantial base substitutions and some small indels but also yielded some novel findings, in particular, novel structural variants in the mitochondrial genomes. These data will enhance the understanding of HIB-induced damage and mutations and aid in the HIB-based microbial mutation breeding.
资助项目	Chinese Academy of Sciences Key Deployment Project[KFZD-SW-109] ; Chinese Academy of Sciences[CAS-ITRI 201801] ; Industrial Technology Research Institute[CAS-ITRI 201801] ; National Natural Science Fund of China[11575259]
WOS关键词	DOUBLE-STRAND BREAKS ; INDUCED DNA-DAMAGE ; ARABIDOPSIS-THALIANA ; MITOCHONDRIAL GENOME ; OXIDATIVE STRESS ; GAMMA-RAYS ; MUTAGENESIS ; SPECTRUM ; IDENTIFICATION ; IMPLANTATION
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000459250200025
资助机构	Chinese Academy of Sciences Key Deployment Project ; Chinese Academy of Sciences ; Industrial Technology Research Institute ; National Natural Science Fund of China
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/64898]
专题	中国科学院近代物理研究所
作者单位	1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 3.Gansu Key Lab Microbial Resources Exploit & Appli, Lanzhou 730000, Gansu, Peoples R China 4.Xinjiang Med Univ, Sch Preclin Med, Dept Pharmacol, Urumqi 830011, Peoples R China 5.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Guo, Xiaopeng,Zhang, Miaomiao,Gao, Yue,et al. A genome-wide view of mutations in respiration-deficient mutants of Saccharomyces cerevisiae selected following carbon ion beam irradiation[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2019,103:1851-1864.
APA	Guo, Xiaopeng.,Zhang, Miaomiao.,Gao, Yue.,Cao, Guozhen.,Yang, Yang.,...&Li, Wenjian.(2019).A genome-wide view of mutations in respiration-deficient mutants of Saccharomyces cerevisiae selected following carbon ion beam irradiation.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,103,1851-1864.
MLA	Guo, Xiaopeng,et al."A genome-wide view of mutations in respiration-deficient mutants of Saccharomyces cerevisiae selected following carbon ion beam irradiation".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 103(2019):1851-1864.