Effects of caffeine cotreatment with radiation on breast cancer susceptibility gene BRCA1

	Effects of caffeine cotreatment with radiation on breast cancer susceptibility gene BRCA1
	Li, Ning1,2,3,4 ; Zhang, Hong1,2,3 ; Wang, Yan-Ling1,2,3 ; Zhou, Xin1,2,3,4 ; Hao, Ji-Fang 1,2,3
刊名	Yuanzineng Kexue Jishu/Atomic Energy Science and Technology
	2011
卷号	45 页码:124-128
ISSN号	1000-6931
英文摘要	The sensitizing effect of caffeine to carbon ion radiation was investigated and the change of BRCA1 expression was observed. The MCF-7 breast carcinoma cells were exposed to carbon ion beams with or without caffeine. The cell survival was automatically monitored by RT-CES system. Cell cycle distribution was assessed by flow cytometry. The levels of BRCA1 mRNA were analyzed by real-time RT-PCR. The expression of BRCA1 protein and its phosphorylation were examined by Western blot. The results show that caffeine increases the sensitivity of MCF-7 cells to carbon ion radiation, and abrogates the radiation-induced G2 arrest. Caffeine inhibits radiation-induced BRCA1 expression both at mRNA and protein level. At the same time, caffeine specifically abolishes BRCA1 phosphorylation of Ser-1524. The data implicate that caffeine inhibits the expression of BRCA1 protein and its phosphorylation.
出版者	Atomic Energy Press
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/64000]
专题	中国科学院近代物理研究所
作者单位	1.Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China 2.Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy of Sciences, Lanzhou 730000, China 3.Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, China 4.Graduate University of Chinese Academy of Sciences, Beijing 100049, China
推荐引用方式 GB/T 7714	Li, Ning,Zhang, Hong,Wang, Yan-Ling,et al. Effects of caffeine cotreatment with radiation on breast cancer susceptibility gene BRCA1[J]. Yuanzineng Kexue Jishu/Atomic Energy Science and Technology,2011,45:124-128.
APA	Li, Ning,Zhang, Hong,Wang, Yan-Ling,Zhou, Xin,&Hao, Ji-Fang.(2011).Effects of caffeine cotreatment with radiation on breast cancer susceptibility gene BRCA1.Yuanzineng Kexue Jishu/Atomic Energy Science and Technology,45,124-128.
MLA	Li, Ning,et al."Effects of caffeine cotreatment with radiation on breast cancer susceptibility gene BRCA1".Yuanzineng Kexue Jishu/Atomic Energy Science and Technology 45(2011):124-128.